DNAzymespaper sensorsRNARNA cleavageEngineering easy‐to 摗爄osensors with ultra﹍ow detection sensitivity remains a major challenge. Herein, we report a simple approach for creating such sensors through the use of an RNAヽleaving DNAzyme (RcD) and a strategy designed to significantly concentrate ...
NA-based catalysis has advanced since seminal work on ribozymes [2] and DNA-based enzymes (DNAzymes) [3]. Unprecedented diversity in the RNA-Cleaving NAzymes in Biosensing The first building block of a typical biosensor is the biorecognition element, used for specific target recognition and ...
The development of the in vitro selection technique has allowed the isolation of functional nucleic acids, including catalytic DNA molecules (DNAzymes), from random-sequence pools. The first-ever catalytic DNA obtained by this technique in 1994 is a DNAzyme that cleaves RNA. Since then, many oth...
replacement, we could considerably enhance the performance of the DNAzyme, demonstrating that the acquired knowledge of the molecular structure, its plasticity and the occurrence of long-lived intermediate states constitutes a valuable starting point for the rational design of next-generation DNAzymes. ...
Both DNAzymes were able to cleave their substrate efficiently in a time- and concentration-dependent manner in vitro. Under a multiple turnover condition, both performed well at 37掳C, showing the kcat of 1 and 0.26 min1, respectively. The phosphorothioate modification of the DNAzyme ...
& Liu, J. In vitro selection of chemically modified DNAzymes. ChemistryOpen 9, 1046–1059 (2020). Article CAS PubMed PubMed Central Google Scholar Silverman, S. K. Catalyic DNA: scope, applications, and biochemistry of deoxyribozymes. Trends Biochem. Sci 41, 595–609 (2016). Article ...
SensitivedualDNAzymes-basedsensorsdesignedbygrafting self-blockedG-quadruplexDNAzymestothesubstratesofmetal ion-triggeredDNA/RNA-cleavingDNAzymes QiZhang 1,a ,YangCai a,1 ,HuiLi a ,De-MingKong a,b,n ,Han-XiShen b a StateKeyLaboratoryofMedicinalChemicalBiology,NankaiUniversity,Tianjin300071,PRChina ...
我们的实验室在试管中建立了用于使 RNA 裂开隔离的选择程序荧光 DNAzymes ( RFDs ;无花果。1) 和调查 RFDs 的使用由于分析 tools.17-29 的 RFDs 在单个的 ribonucleotide 连接刺激一 DNA-RNA 荒诞不经的 substrate 的分裂 (R) 那被一 fluorophore 侧面与相接 (F) 和一个熄灭者 (Q)。F 和 Q 的接近的接...
RNA cleaving '10-23' DNAzymes with enhanced stability and activity. 10-23' DNAzymes can be used to cleave any target RNA in a sequence-specific manner. For applications in vivo, they have to be stabilised against nucleolyti... S Steffen,DC Gül,G Hans‐Peter,... - 《Nucleic Acids Re...
C. DNA enzymes as potential therapeutics: towards clinical application of 10-23 DNAzymes. Expert Opin. Biol. Ther. 15, 689–711 (2015). Article CAS Google Scholar Krug, N. et al. Allergen-induced asthmatic responses modified by a GATA3-specific DNAzyme. N. Engl. J. Med. 372, 1987...