MiRNA biogenesis is highly regulated at the post-transcriptional level; however, the role of sequence and secondary RNA structure in this process has not been extensively studied. A single G to A substitution present in the terminal loop of pri-mir-30c-1
structure. This suggests that ADAR1 and A-to-I editing, mediated by MDA5 polymerization to dsRNA and MAVS activation, play a crucial role in suppressing IFN production and preventing aberrant innate immune responses [24,33,84] (Fig.5). Fig. 5 The RNA editing enzyme ADAR1 plays a crucial ...
a, A density plot showing the distribution of structural heterogeneity at a transcript level for the hESCs. Red dashed lines indicate 25% percentile (most homogeneous) and 75% percentile (most heterogeneous) of transcripts. The names of selected genes are labeled, and 18S rRNA is labeled in red...
b Distribution of F1 score for individual RNAs on the independent test set TS1 given by various methods as labeled. On each box, the central mark indicates the median, and the bottom and top edges of the box indicate the 25th and 75th percentiles, respectively. The outliers are plotted ...
Fig. 1.Common structural domains shared by members of the hnRNP family. A schematic diagram is given showing the protein structure with the N- (NH2) and C- (COOH) termini labeled. All members contain at least one nucleic acid binding domain which is able to bind DNA and/or RNA in a ...
We labeled MDA-MB-231 cell-derived exosomes with PKH26 and incubated them with macrophages to examine exosome incorporation and confirmed that the labeled exosomal RNAs could be internalized by macrophages (Fig. 6h). Then, we cocultured unpolarized macrophages with exosomes isolated from lncRNA B...
The large 50 S ribosomal subunit (yellow) is shown to the rear of this view with the L1 stalk and the L7/L12 regions labeled. The small 30 S subunit is shown to the front, but made transparent so as not to obscure the aminoacyl (A), peptidyl (P) and exit (E) tRNAs, as well ...
(NEB) ligated to the 3′ end of RNA was 5′ labeled with T4 polynucleotide kinase (NEB) and γ-P32-ATP. 1 µL of the 5′ labeled primer (~250 nM) was annealed to the resuspended RNA samples at 95 °C and slowly cooled down to ~40 °C. Finally, primer extension was...
The nucleoprotein (NP) of Marburg virus (MARV), a close relative of Ebola virus (EBOV), encapsidates the single-stranded, negative-sense viral genomic RNA (vRNA) to form the helical NP–RNA complex. The NP–RNA complex constitutes the core structure for
The stop codon and anti-stop codon (i.e., base-paired with the stop codon) are labeled below the multiple alignments. For this structure, 8.8% of all potential base pairs are one- or two-sided covariants. (E and F) Nearly half of the human genome is comprised of repetitive elements,...