The SARS-CoV-2 spike is most similar in sequence and structure to SARS-CoV spike protein12, and shares a global protein fold architecture with the MERS-CoV spike protein (Fig.1) allowing us to build on our prior vaccine construct design10. Unlike glycoproteins of HIV and influenza, the pre...
For the stable RT and USP7 RT, synchronously cycling cells were pulse labeled with the nucleotide analog BrdU for 2 h, respectively. Cells were sorted into early, mid and late S-phase fractions, 20,000 cells each, on the basis of DNA content using FACS. For the transitional RT, existi...
In parallel, we isolated spontaneously generated GFP-labeled single CTCs and CTC clusters from three mouse xenograft models, including two human breast CTC-derived cell lines (BR16 and BRx50) and the human breast cancer cell line MDA-MB 231 (lung metastatic variant, referred to as LM2) (...
DNA metabarcoding had a good resolution in detecting MT at the species level and provided insights into the total species composition of herbal preparations labeled as containing unique (U) and multi-ingredients (M). The DNA metabarcoding and LC-MS semi-targeted metabolomics results were in accordan...
Labeled peptides were combined, acidified with FA (pH ∼2) and dried via vacuum centrifugation. For fractionation the high pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific) was used. The peptides were resuspended in 0.1% TFA and separated into seven fractions with increasing...
4A). To confirm ADP ribosylation of BORIS, an in vitro ADP ribosylation assay was performed using the purified BORIS-N1-258 protein and biotin-labeled PAR polymers. Biotin-labeled PAR bound to BORIS-N1-258 in a concentration-dependent manner in vitro (Fig. 4B). Next, H1299 cells ...
(1). Reads are labeled with their corresponding barcode, reads are aligned uniquely to the reference genome, PCR duplicates are removed by alignment and UMI, alignments are strand separated, and alignments are compared to a list of authentically expressed L1 loci from a bulk RNA-Seq dataset in...
The device labeled "other delivery channels" represents traditional presentation devices, such as client workstations, Interactive Voice Response Units (IVRUs), or specialized input devices such as point-of-sale terminals or ATMs. This model has increasing complexity as it scales the number of ...
The device labeled "other delivery channels" represents traditional presentation devices, such as client workstations, Interactive Voice Response Units (IVRUs), or specialized input devices such as point-of-sale terminals or ATMs. This model has increasing complexity as it scales the number of ...
3 Zinc finger domain of YY1 is essential, but not sufficient for the specific binding toward G4 structure. a, A schematic diagram depicting the domain structure of YY1 protein. b-e, Fluorescence anisotropy for monitoring the bindings of mutant (YY1C360S) and truncated (YY11-382, YY1231-...