MiRNA biogenesis is highly regulated at the post-transcriptional level; however, the role of sequence and secondary RNA structure in this process has not been extensively studied. A single G to A substitution present in the terminal loop of pri-mir-30c-1 in breast and gastric cancer patients ...
T7 DNA Pol can be used in a manner similar to T4 DNA Pol for labeling 3′ termini either by simple extension or by replacement synthesis in the presence of radiolabeled or nonisotopically labeled dNTP(s). 3. STRUCTURE i. Subunit assembly. T7 DNA Pol is a dimeric enzyme: a p...
. This resulted in seven domains (labeled I–VII in Table1) likely to generate structured RNAs and 11 individual windows with z-scores less than −1.70. Concatenated domains range from 160 to 280 nt (comprising two to six overlapping windows). Interestingly, in addition to their low z-scor...
Fig. 9.Zone sedimentation sucrose gradient analysis of3H-labeled heterogeneous nuclear RNAs of control (CHn) and irradiated (XHn) cells labeled with3H-labeled 5-uridine for 60 min in the presence of 0.05 Mg/ml actinomycin D. Identical aliquots of cytoplasmic14C-labeled RNA (▴) were added ...
Cells read only those genes which are needed at a given moment, while the others are chemically labeled and, thus, selectively turned off. Scientists at the German Cancer Research Center have now been the first to discover how these labels are placed at exactly the right spot in the genetic...
3.0.CO;2-Y/asset/image_m/mcontent.gif?v=1&s=0b3a282c61d30802ffa11689bf8eba508d7313ab" class="figZoom" shape="rect">In the living cell is where the authors hope to elucidate the structure of RNA by using their bispyrene-conjugated 2′-O-methyloligoribonucleotide (OMUpy2-I) ...
By incorporating unlabeled data, the model can learn more about the underlying patterns and structure of the data, leading to better generalization. This approach is particularly useful when acquiring labeled data is costly or time consuming, as it can make the most of available resources and ...
In short, 6.25 nM biotin-labeled IRE RNA was incubated with 2 μg of cytosolic liver extract with or without 1 μM of unlabeled IRE RNA. The result of the assay control is presented on Supplementary Figure S6. Binding, electrophoresis and detection of the tested RNAs with the proteins were...
B, SAS, and AFM can provide intermediate-to-low resolution 3D envelope, shape or topography structure for RNA. C, EPR, XSI, and smFRET are three molecular rulers that can provide pair-wise distance distributions for RNAs in the nanometer range, where the RNAs are site-specifically labeled ...
Formally, an RNA sec- ondary structure is a (labeled) graph whose nodes repre- sent nucleotides. The edge set contains edges between consecutive nodes (i, i + 1) representing the phosphate backbone as well as edges between base pairs. For the latter, the following conditions must hold: 1...