RNA-seqSingle-cell RNA-seqWnt signalingThe goal of this paper is to review state-of-the-art transcriptome profiling methods and their recent applications in the field of skeletal biology. Next-generation sequencing of mRNA (RNA-seq) methods have been established and routinely used in skeletal ...
We employed next-generation RNA sequencing (RNA-Seq) technology to determine the influence of obesity on global gene expression in skeletal muscle feed arteries. Transcriptional profiles of the gastrocnemius and soleus muscle feed arteries (GFA and SFA, respectively) and aortic endothelial cell-enriched...
ScRNA-seq has four major advantages over bulk RNA-seq in biological research. First, this approach can reveal cellular heterogeneity or specific subclusters; second, it can depict the trajectories of different cell states, transitions, and differentiation in tissue during physiological and pathological ...
Skeletal muscle development is a multistep process whose understanding is central in a broad range of fields and applications, from the potential medical value to human society, to its economic value associated with improvement of agricultural animals. Skeletal muscle initiates in the somites, with mu...
Therefore, large scale genome-wide molecular phenotyping of MSCs from diverse tissues is to properly identify these cells and understand their biology. RNA sequencing (RNA-seq) using next-generation-sequencing (NGS) platforms has greatly improved the analysis of whole transcriptomes, allowing for the ...
Single-cell RNA sequencing (scRNA-seq), a technology that analyzes transcriptomes of complex tissues at single-cell levels, can identify differential gene expression and epigenetic factors caused by mutations in unicellular genomes, as well as new cell-specific markers and cell types. scRNA-seq plays...
All RNA-seq data were aligned to hg19 using TopHat[2] v2.0.9 with default parameters. The mapped reads were assembled using Cufflinks[3] v2.11. All multiple assembled transcript files (GTF format) were then merged to produce a unique set of transcriptomes using the Cuffmerge utility ...
In the present study, we analyzed the RNA-seq data of human meniscus samples from patients with knee OA and healthy individuals. OA-associated DEGs were observed to be significantly enriched in the signaling pathways related to extracellular matrix organization and ossification. Meanwhile, OA-associate...
this method does not reveal the identity and function of specific cellular subsets or the differences in gene expression at the single-cell level,10which limits our understanding of the biology and pathology of these diseases. Single-cell RNA sequencing (scRNA-seq) allows researchers to investigate...
[25]. In these mice the expression of Cre recombinase is controlled by the promoter of theMyf5gene, which is almost exclusively expressed in skeletal muscle tissue. Therefore, expression ofGrsf1is abrogated in skeletal muscle tissue of these mice. Interestingly, the authors found that at higher ...