Using the toolRNA-seq strandedness inference and inner distance estimation using RseQC:We added this tool under theQuality controlcategory to help you. The tool aligns subsets of the input FASTQ files against the reference genome, and this alignment is then compared to the reference annotation to ...
Rachelle HerrinCeleste EngWilliam J. JanssenAndrew H. LiuSam S. OhRajesh KumarBioMed CentralWesolowska-Andersen A, Everman JL, Davidson R, Rios C, Herrin R, Eng C, et al. Dual RNA-seq reveals viral infections in asthmatic children without respiratory illness which are associated with changes...
[6,7,8]. However, it has to be said that the epigenetic mechanisms and therapies in maintaining the aqueous humor outflow remain to be defined. N6-methyladenosine (m6A) RNA modification is the most conserved and predominant internal mRNA modification found in current studies. It is one of ...
We found that escalating doses of paclitaxel resulted in multinucleation, promotion of senescence, and initiation of DNA damage induced apoptosis. Single-cell RNA sequencing (scRNA-seq) of TNBC cells after paclitaxel treatment revealed upregulation of innate immune programs canonically associated with ...
Reference transcriptomes can be constructed through de novo assembly of RNA sequencing (RNA-Seq) data [19]. RNA-Seq, a promising application of next-generation sequencing (NGS) [20], is a cost-effective and widely utilized strategy [21] that has been successfully applied to analyze entire ...
We found that escalating doses of paclitaxel resulted in multinucleation, promotion of senescence, and initiation of DNA damage induced apoptosis. Single-cell RNA sequencing (scRNA-seq) of TNBC cells after paclitaxel treatment revealed upregulation of innate immune programs canonically associated with ...
We were particularly interested in the auditory modulation at swarm time. Swarm formation in mosquitoes is a rhythmic behaviour under circadian regulation. In the final step of our RNA-seq analysis, we identified transcripts with a rhythmic (orcycling) expression in the 24-hour period of data col...
(Korea and The Netherlands) using the same primer combinations as for PCR, except for Am-β-tub-F, which was replaced by the shorter primer Am-β-tub-F-Seq (5’-CGGAGCRGGTAACAAYTG-3’) following Thongbai et al. (2017). The sequences were assembled in Geneious Pro v. 6.0.6 (Bio...
Although they are found in next-generation whole transcriptome sequencing (RNA-Seq) data on a regular basis, investigating them further has usually been refrained from. Therefore, their expression patterns or functions in general, and in oncogenesis in particular, are poorly understood. Results: We...
RNA-seq, using total RNA, was utilized to determine transcriptomic alterations within the liver.The HFCF diet led to a sequential development of liver damage in mice, starting with steatosis, evolving into early steatohepatitis, escalating to steatohepatitis with fibrosis, and ultimately culminating in...