6 FILES Notice:choose FTP method to upload data; thenclick “FTP upload instructions”, get the account and password for uploading. Download and install an FTP software:Filezilla (https://filezilla.software/download/), or other software you like Fill in the Address, Username, Password get from...
用RNA-SEQ测一个新的物种的深度测序，然后分析，可以发什么样的文章？？？现在缺5左右的文章 好多SCI...
Normal RNA-Seq libraries are designed to randomly sequence any part of a transcript which may not always be representing the identity of each transcript, meaning that multiple locus in a transcript should be sequenced to identify a transcript. However, if you can read exactly the same locus of...
The authors used mRIN to quantify degradation in large-scale RNA-seq data from postmortem brain tissue in the BrainSpan and Genotype-Tissue Expression (GTEx) projects and found that degradation has a reproducible gene-specific component. This is a preview of subscription content, access via your ...
RNA-seq helps determinegene expressionpatterns based on the number of reads that map to each transcript sequence. It provides comprehensive transcriptome profiling, allowing researchers to detect and quantify the expression of both protein-coding and non-coding RNA molecules. ...
Token file is necessary to be able to download controlled data on your server Search for data you have access and you want to work on in the search box on the main page. Choose 'RNA-Seq' under experimental strategy section Go to 'Files' section on the left-hand side of the page and...
RSEM1,2 is an RNA-Seq transcript quantification program developed in 2009. In this tutorial, we will use some single cell RNA-Seq data from Shalek et al. to demonstrate the common uses of RSEM.The Shalek et al. study contains thousands of single cell RNA-Seq experiments from bone-marrow-...
I have RNA-Seq data which is paired-end reads. Extracted the counts using featureCounts for all the samples. There is a function to convert counts to RPKM: using the gene_length rpkm <- function(counts, lengths) { rate <- counts / lengths rate / sum(counts) * 1e6} ...
You can directly submit ribosomal RNA (rRNA), rRNA-ITS, or Influenza sequences to GenBank. Other sequence types should be submitted with one of the alternative tools. For unassembled raw sequence reads, you can submit them to the Sequence Read Archive (SRA). ...
Hi, I'm new to single cell RNA sequencing, and I wanted to reproduce the results in a published dataset (Tirosh, et al, Dissecting the multicellular ecosystem of metastatic melanoma by single-cell RNA-seq, Science, 2016). The only data I currently have access to an integrated dataset file...