How to Analyze Gene Expression Using RNA-Sequencing Data. In Next Generation Microarray Bioinformatics: Methods and Protocols; Wang, J., Tan, A.C., Tian, T., Eds.; Humana Press: New York, NY, USA, 2012; pp. 259-274.Ramskold D, Kavak E, Sandberg R (2012) How to analyze gene ...
How to Find Desired Logs in This Manual When a device is running, the log module on the device records user operations and events into log messages. Log messages allow you to learn about the running status of the device, analyze the network situations, locate faults, as well as diagnose an...
As RNA-seq has advanced, it has been coupled with LCM to enable gene expression studies at the single-cell level. The RNA-seq-LCM combined technique has been used to characterize transcriptomes of different types of phloem in cucumber (Cucumis sativus) fruit during early plant development [96,...
[95]. A study to analyze the expression levels of different types in GBM found that, CALM1,and CALM2 are significantly expressed more than CALM3 in GBM. Utilizing data from Gene Expression Profiling Interactive Analysis, it was found that Ca+2calmodulin gene expression was higher in GBM and...
What typically took months in the past on traditional infrastructure, Caris accomplished in just 2.5 days using AWS. This breakthrough enabled them to analyze23,000 RNA genes per samplewhile managing their extensive 40+ PetaByte multimodal database. ...
In this tutorial, we first analyze one paired-end RNA-Seq data set from a single dendritic cell sequenced 6 hours after lipopolysaccharide (LPS) stimulation. Then we demonstrate how to perform differential expression analysis on two groups of single cells. Lastly, we show how we can use the ...
In this tutorial, we first analyze one paired-end RNA-Seq data set from a single dendritic cell sequenced 6 hours after lipopolysaccharide (LPS) stimulation. Then we demonstrate how to perform differential expression analysis on two groups of single cells. Lastly, we show how we can use the ...
It goes beyond just getting the protocol to work: optimizing the protocol for your specific needs, making sure everyone is trained on it, and learning how to analyze new kinds of data are non-trivial aspects when using single cell sequencing....
We will analyze the benefits of using different RNA-Seq approaches and provide the best practices for FFPE RNA-Seq. Let’s start by exploring the details of FFPE sample preparation and potential challenges and precautions to take. And…stay tuned for more updates! How to prepare FFPE samples?
With the intensification of the greenhouse effect, a series of natural phenomena, such as global warming, are gradually recognized; when the ambient temperature increases to the extent that it causes heat stress in plants, agricultural production will in