This data set allowed us to analyse gain and loss of transcripts and ORFs in short evolutionary timescales (Supplementary section 6.2, Figure S6). If an asORF is found in at least one line, it is gained once in D. melanogaster. More specifically, the most recently emerged asORF would be...
Normally, the sequence data from Whole Transcriptome Sequencing comprises the data for expression profiling as well, and that is why it is the most common form of RNA-Seq projects at the moment. If you simply order an RNA-Seq library prep kit without specifying your objective, you will most...
We will analyze the benefits of using different RNA-Seq approaches and provide the best practices for FFPE RNA-Seq. Let’s start by exploring the details of FFPE sample preparation and potential challenges and precautions to take. And…stay tuned for more updates! How to prepare FFPE samples?
I’m starting a final year undergraduate project as of this week, on mapping small RNA-seq data tomiRNAs, under the supervision of the founder ofthe databasecentral to cataloguing this class of non-coding RNA ‒ super exciting stuff! :¬) If you’ve got questions on PISA you think I...
[13] on single-cell RNA sequencing (scRNA-Seq) data focused on evaluating the performance of a set of methods for missing value imputation proposed in different contexts, when applied to the scRNA-Seq field. In particular, the authors aimed at verifying if some of the tested tools were ...
As expected, whole genome sequencing (WGS) and RNA-Seq were the most common methods used to obtain genomic and transcriptomic data (Fig. 1). For genomic data, we observed a significant number of BioProjects which were tagged with epigenomic type studies performed with ChIP-Seq, BiSulfite-Seq,...
therapeutic implications [162]. Whole-Genome Bisulfite Sequencing (WGBS) offers a comprehensive DNA methylation profile in a broad range of biological systems. It can be used to analyse single-cell methylomes, whereas ChIP-seq allows the study of histone modifications on the single-cell level [163...
In this study, we aimed to clarify the complex mechanisms between clinical medicine and ferroptosis in cancer cells and to analyse the feasibility of targeting ferroptosis to improve clinical treatment strategies and to provide new ideas for subsequent clinical treatment. 3. Antitumour treatment drugs ...
[13] on single-cell RNA sequencing (scRNA-Seq) data focused on evaluating the performance of a set of methods for missing value impu- tation proposed in different contexts, when applied to the scRNA-Seq field. In particular, the authors aimed at verifying if some of the tested tools were ...
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