在很久很久以前,年轻的Piotr Chomczynski老爷爷和Nicoletta Sacchi老奶奶在美国NIH大院里相遇,互相感慨科研经费不好拿啊,仪器设备都贵啊,咱得省着点花钱啊,于是两人一合计,发明了简单便宜的TRizol抽提RNA的方法(Single-step method of RNA isolation by acid guanidinium thi...
4.3.4RNA Isolation This section describes a standard protocol that is used for sequencing ofRNA virusesat the D.I. Ivanovsky Institute ofVirologyin Moscow, Russia. The most appropriate method for thepurificationof RNA for subsequent sequencing is the use of a TRIzol® reagent. ForRNA isolation...
During sample homogenization or lysis, TRIzol® Reagent helps to maintain the integrity of the RNA while disrupting cells and dissolving cell components. The MagMAX™-96 Total RNA Isolation Kit utilizes MagMAX™ technology to purify total RNA on magnetic beads in a tube or...
在很久很久以前,年轻的Piotr Chomczynski老爷爷和Nicoletta Sacchi老奶奶在美国NIH大院里相遇,互相感慨科研经费不好拿啊,仪器设备都贵啊,咱得省着点花钱啊,于是两人一合计,发明了简单便宜的TRizol抽提RNA的方法(Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. 1987. ...
During sample homogenization or lysis, TRIzol® Reagent helps to maintain the integrity of the RNA while disrupting cells and dissolving cell components. The MagMAX™-96 Total RNA Isolation Kit utilizes MagMAX™ technology to purify total RNA on magnetic beads in a tube...
TRIZOL RNA Isolation ProtocolChomczynski PMackey KChomczynski, P., Mackey, K., 1995. TRIzol RNA isolation protocol. BioTechniques 19 (6), 942-945.
Briefly, the RNA was isolated using trizol method. Then 1.5 µg of it is converted to cDNA using High Capacity cDNA reverse transcription kit. The cDNA was then mixed with gene specific primers (mSLC4A8: F-CGATTGAGTCGTTGTTTGGA, R-AAGGGCATAGTCCTTGCAGA; mRNF20: F-TTGAACTCCTTCCTTG...
DNA_RNA_Isolation_TRIZOL DNA Isolation: TRIZOL Reagent 1. Tissues: KEEP FROZEN on dry ice, Homogenize in 1 mL Trizol, under Fume Hood using tissue homogenizer.a)Put probe on drill.b)Set up epi tubes in rack.c)Dislodge tissue form cryotube with forceps, tap into homogenizer tube, throw ...
TRIZOLTM Reagent is an improved version of the single-step RNA isolation method.TRIZOL Reagent (patent pending) is a monophasic solution of phenol and guanidine isothiocyanate. During sample homogenization or lysis, TRIZOL Reagent maintains the integri- ty of the RNA, while disrupting cells and ...
Cells were harvested and total RNA was isolated using TRIzol (Thermo Fisher). Isolation of small RNAs from total RNA Samples was done with themirVana miRNA Isolation Kit (Thermo Fisher). Complementary DNA (cDNA) synthesis was performed after DNase treatment (TURBO DNA-free Kit, Thermo Fisher) ...