In order to investigate the reproducibility of each method, collected supernatant was then divided into three equal volumes, i.e. 600 μl. Total cellular RNA Isolation, RNA Quantity, RNA purity, and RNA Quality Measurement Collected supernatant was directly applied on the RNeasy column (SGC; ...
RNA modifications play a pivotal role in the regulation of RNA chemistry within cells. Several technologies have been developed with the goal of using RNA modifications to regulate cellular biochemistry selectively, but achieving selective and precise mo
for using Triton X-100 method. With the same principle, modified Trizol method, modified RNAiso plus method and Triton X-100 method reported here can be employed for extraction of RNA that are free from 16S and 23S rRNA and provide simple, rapid and effective tools for the isolation of ...
RNA isolation. TRIzol reagent (Life Technologies) was employed to isolate total RNAs from the cells and xenograft tissues. In brief, cells or gridded tissues were lysed in the mixture with 1 ml TRIzol reagent and 100 μl 1-bromo-3-chloropropane (BCP) (Molecular Research Center) by...
for using Triton X-100 method. With the same principle, modified Trizol method, modified RNAiso plus method and Triton X-100 method reported here can be employed for extraction of RNA that are free from 16S and 23S rRNA and provide simple, rapid and effective tools for the isolation of ...
Cell pelleting, lysis and RNA isolation was performed as described above (Small RNA SLAMseq in Drosophila S2 cells). 50 μg total RNA was fragmented in 20 μl 1x RNA Fragmentation buffer for 1 min at 94°C (NEBNext® Magnesium RNA Fragmentation Module), resulting in ∼1 kb fragments...
内容提示: Isolation of Total RNA from Animal Tissues and Gel Electrophoresis I.Objectives 1.Master the principle, methods of RNA isolation and detection; 2.Learn some related techniques for handling RNA. II.Theory 1. Brief introduction of RNA RNAs are a sort of central biological molecules which...
RNA was isolated using Trizol extraction as previously described33. FF tissue Using the PicoPure® RNA Isolation Kit (Cat.No. KIT0204; Thermo Fisher Scientific) according to manufacturer’s instructions, the dissected rat and human tissues were lysed for 30 minutes at 42 °C and RNA was...
In this method, dUTPs are incorporated only into the second strand of cDNAs during cDNA synthesis and these dUTPs are then excised prior to library amplification, ensuring that only the first cDNA strand is efficiently amplified. Incomplete incorporation and/or excision of dUTPs would presumably ...
2.3 mRNA/RNA Isolation, miRNA-RT-PCR and Global Transcriptome Analysis RNA isolation was done with TRIzol reagent (Invitrogen, Germany) or the mirVana miRNA Isolation Kit (Ambion, USA) according to manufacturers' instructions. For detection of miRNAs in samples different TaqMan MicroRNA assays (Appl...