Notably, the choice of the technique for RNA isolation had a significant impact on the potential to obtain appropriate copy numbers of bigger fragments as was demonstrated for the 1400 bp fragment of PBGD. In this respect, the SGC technique proved to be superior to the GTC technique both in...
RNA isolation A Direct-zol RNA MicroPrep kit (Zymo Research) was used to isolate total RNA following the manufacturer’s instructions. DNA digestion was performed on the column at room temperature for 15 min to remove the DNA contamination of the extracted RNA. For a larger scale of RNA purif...
Formalin-fixed paraffin-embedded (FFPE) tissues are valuable resources commonly used in pathology. However, formalin fixation modifies nucleic acids challenging the isolation of high-quality RNA for genetic profiling. Here, we assessed feasibility and reliability of microarray studies analysing transcriptome ...
RNA isolation, qRT-PCR Total RNAs from cultured cell lines or mouse tissues were extracted with Trizol Reagent (Invitrogen) according to the manufacturer’s protocol. The reverse transcription was carried out using PrimeScript RT Master Mix (TaKaRa, Japan) according to the manufacturer’s protocol...
[100], or in the case of nascent RNAs using chromatin isolation [101], the ability to capture simultaneously all reads with an existing current Nanopore sequencing technique is still not present. However, as the methodology of direct RNA sequencing matures and additional library approaches, such ...
RNA isolation. TRIzol reagent (Life Technologies) was employed to isolate total RNAs from the cells and xenograft tissues. In brief, cells or gridded tissues were lysed in the mixture with 1 ml TRIzol reagent and 100 μl 1-bromo-3-chloropropane (BCP) (Molecular Research Center) by...
In principle, small RNAs that originate from symmetric duplexes should display compound half-life kinetics to account for the dual fate of each strand (i.e., acting partially as stable guide or unstable miR∗). Indeed, by carefully inspecting small RNA decay, we found that six out of 18...
内容提示: Isolation of Total RNA from Animal Tissues and Gel Electrophoresis I.Objectives 1.Master the principle, methods of RNA isolation and detection; 2.Learn some related techniques for handling RNA. II.Theory 1. Brief introduction of RNA RNAs are a sort of central biological molecules which...
RNA Isolation, RT-PCR, and qPCR Total RNAs were purified using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. cDNAs were synthesized from total RNAs (1 μg) using the DiaStar™ cDNA synthesis kit (SolGent, Korea). To confirm the expression of Factor VIII, Brachyury...
2.3 mRNA/RNA Isolation, miRNA-RT-PCR and Global Transcriptome Analysis RNA isolation was done with TRIzol reagent (Invitrogen, Germany) or the mirVana miRNA Isolation Kit (Ambion, USA) according to manufacturers' instructions. For detection of miRNAs in samples different TaqMan MicroRNA assays (Appl...