前(图1)无明显差异,用DNase处理后,仍有杂带,无明显改变,在最下1条亮带以上,能清晰看到4条条带,这与正常RNA电泳条带相异,推测Omega试剂盒不适合发菜藻体RNA的提取;改良后的Trizol法23S、16S 2条带已有大部分降解,5S条带比较明亮,并伴有拖尾现象,表明该方法所提RNA降解快,不适合进行后续试验(图5);优化后的...
Cell pellets were then lysed in TRIZOL and RNA extracted according to manufacturer’s instructions. The purified RNA was then subjected to cDNA synthesis and quantitative real-time PCR analysis in a single reaction, using the Power SYBR Green RNA-to-CT 1-Step kit. The QuantStudio 7 Flex ...
RNA isolation. TRIzol reagent (Life Technologies) was employed to isolate total RNAs from the cells and xenograft tissues. In brief, cells or gridded tissues were lysed in the mixture with 1 ml TRIzol reagent and 100 μl 1-bromo-3-chloropropane (BCP) (Molecular Research Center) by...
Trizol Invitrogen Cat# 15596026 human GM-CSF Milteny Biotec Cat# 130-093-864 interleukin3 (rhIL-3) Milteny Biotec Cat# 130-093-909 interleukin6 (rhIL-6) Milteny Biotec Cat# 130-093-931 stem cell factor (rhSCF) Milteny Biotec Cat# 130-096-694 thrombopoietin (rhTPO) Milteny Biotec...
Then total RNA was extracted with TRIzol LS reagent, and resuspended in 20 μl RNase-free water. The RNA was fragmented by base hydrolysis as follows: The RNA was denatured at 65°C for 40s, immediately cooled on ice, and added with 5 μl of 1 M NaOH for 10 min on ice. Then, ...
RNA was isolated using Trizol extraction as previously described33. FF tissue Using the PicoPure® RNA Isolation Kit (Cat.No. KIT0204; Thermo Fisher Scientific) according to manufacturer’s instructions, the dissected rat and human tissues were lysed for 30 minutes at 42 °C and RNA was...
RNA isolation, cDNA synthesis and PCR experiments Total RNA was isolated using either Trizol (Invitrogen) or RNeasy kit (QIAGEN, Valencia, CA, USA) according to the manufacturer's instructions. The concentration of RNA was determined using a spectrophotometer and Experion RNA StdSens (BIO-RAD, ...
Total RNA was extracted from samples using Trizol reagent (Life Technologies, USA) and converted to complementary DNA (cDNA) using the PrimeScript RT Reagent Kit (Vazyme, China) on a 96-Well Thermal Cycler (Applied Biosystems, USA). Quantitative PCR (qPCR) was conducted with the One-Step TB...
RNA was isolated by incubation by TRIzol (Invitrogen) followed by passage through Direct-zol columns (Zymo Research). Isolation of M. tuberculosis RNA included bead beating during incubation with TRIzol [33]. Total RNA was depleted of ribosomal RNA using the Ribo-Zero rRNA Removal Gram-negative ...
Praveen Mamidala, Loren J Rivera-Vega & Omprakash Mittapalli Molecular and Cellular Imaging Center, The Ohio State University, Ohio Agricultural and Research Development Center, Wooster, OH, 44691, USA Asela J Wijeratne, Saranga Wijeratne & Tea Meulia Division of Sensory Biophysics, The Ohio Sta...