提取总rna用trizol试剂rna提取试剂盒invitrogen公司卡尔斯巴德ca美国从表皮生长因子刺激100纳克毫升细胞的各种长度的时间结果一 题目 英语翻译Total RNA was extracted using a Trizol RNA extraction kit(Invitrogen,Carlsbad,CA,USA) from cells stimulated with EGF (100 ng/mL) for various lengths of time.The first...
aFor COC and GC samples, total RNA was isolated using TRIzol (Invitrogen Australia Pty. Ltd.) per the manufacturer’s instructions, with the inclusion of 15μg of Blue Glycogen (Ambion) during precipitation. 为COC和GC样品,总RNA使用TRIzol Invitrogen (澳洲Pty被隔绝了。 每) 制造商的指示的有限...
Total RNA Isolation Using Trizol and Qiagen RNAeasy ColumnsRequired, Materials
总RNA 是从一种同时试剂细胞中提取的。 翻译结果4复制译文编辑译文朗读译文返回顶部 总RNA的对待是节录自使用trizol细胞试剂。 翻译结果5复制译文编辑译文朗读译文返回顶部 总RNA从被对待的细胞被提取了使用TRIzol试剂。 相关内容 a你怎样来学校的 How do you come the school [translate] aone car come,one car ...
Total RNA isolated using TRIZOL was contaminated with genomic DNA but treatment with enzyme DNase, in solution or on-column, efficiently removed the genomic DNA. Furthermore, conditions for elimination of genomic DNA contamination on-column and isolation of pure total RNA from leaves were optimized....
总RNA提取是从细胞和组织ffpe使用trizol试剂[Invitrogen在]。 我们合成一步合作使用primescript??? 自然资源部综合套件[合作]宝工业。 成熟的表达式的"和平"号轨道是由214sybr绿色定量实时PCR放大在ABI7500超线程(HT)文书[应用生物系统公司]。 用于数据分析、循环阈值[CT]U6[参考]和MIR214[示例]是在一九六八年四月确...
进行Total RNA提取步骤：1.使用枪吸去细胞培养液，随后用1xPBS洗一次。2.加入1ml TRIzol，用枪反复吹吸至溶液不再粘稠，将溶液转移至1.5mlEP管中。3.在常温（15℃~30℃）下静置5min，以确保核蛋白复合体充分裂解。4.加入270ul氯仿，剧烈振荡15s，于常温（15℃~30℃）下静置15min。5.离心4℃...
Total RNA was extracted from cells using TRIzol reagent (Thermo Fisher Scientific, Inc.) according to the manufacturer’s protocol. A total of 2 μg of RNA was reverse-transcribed into cDNA using a ReverTra Ace qPCR RT Kit (Toyobo Life Science, Osaka, Japan) according to the manufacturer...
确保所有化学试剂，包括PBS、Trizol或其它RNA提取试剂，都是新鲜且未被污染。6.快速均质化:组织破碎和...
Total RNA were extracted from the samples of liver (approximately 50 mg), kidney (approximately 50 mg), lung (approximately 50 mg) and intestine (approximately 50 mg) were extracted using Trizol reagent (Applied Biosystems, Foster City, CA, USA) according to the protocol provided by the manufa...