提取总rna用trizol试剂rna提取试剂盒invitrogen公司卡尔斯巴德ca美国从表皮生长因子刺激100纳克毫升细胞的各种长度的时间结果一 题目 英语翻译Total RNA was extracted using a Trizol RNA extraction kit(Invitrogen,Carlsbad,CA,USA) from cells stimulated with EGF (100 ng/mL) for various lengths of time.The first...
aFor COC and GC samples, total RNA was isolated using TRIzol (Invitrogen Australia Pty. Ltd.) per the manufacturer’s instructions, with the inclusion of 15μg of Blue Glycogen (Ambion) during precipitation. 为COC和GC样品,总RNA使用TRIzol Invitrogen (澳洲Pty被隔绝了。 每) 制造商的指示的有限...
Total RNA Isolation Using Trizol and Qiagen RNAeasy ColumnsRequired, Materials
Total RNA isolated using TRIZOL was contaminated with genomic DNA but treatment with enzyme DNase, in solution or on-column, efficiently removed the genomic DNA. Furthermore, conditions for elimination of genomic DNA contamination on-column and isolation of pure total RNA from leaves were optimized....
总RNA 是从一种同时试剂细胞中提取的。 翻译结果4复制译文编辑译文朗读译文返回顶部 总RNA的对待是节录自使用trizol细胞试剂。 翻译结果5复制译文编辑译文朗读译文返回顶部 总RNA从被对待的细胞被提取了使用TRIzol试剂。 相关内容 a你怎样来学校的 How do you come the school[translate] ...
进行Total RNA提取步骤：1.使用枪吸去细胞培养液，随后用1xPBS洗一次。2.加入1ml TRIzol，用枪反复吹吸至溶液不再粘稠，将溶液转移至1.5mlEP管中。3.在常温（15℃~30℃）下静置5min，以确保核蛋白复合体充分裂解。4.加入270ul氯仿，剧烈振荡15s，于常温（15℃~30℃）下静置15min。5.离心4℃...
Trizol Total Rna Isolation Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/trizol total rna isolation reagent/product/Thermo Fis...
The ETEC total RNA was isolated using a Bacteria RNA Extraction Kit (Vazyme, China) according to the manufacturer’s instructions. The IPEC-J2 cells were stimulated with 40 μg ETEC total RNA delivered by lipofectamine 2000 (Invitrogen, USA) for 8 h. For the negative control, the same ...
Total RNA were extracted from the samples of liver (approximately 50 mg), kidney (approximately 50 mg), lung (approximately 50 mg) and intestine (approximately 50 mg) were extracted using Trizol reagent (Applied Biosystems, Foster City, CA, USA) according to the protocol provided by the manufa...
英文名称Trizol (Total RNA Extractor Reagent) 储存温度2-8°C储存,避免反复冻融 运输条件冰袋运输 产品介绍 产品介绍: TRzol试剂是直接从细胞或组织中提取总RNA的试剂。它在破碎和裂解细胞时能保持RNA的完整性。加入氯仿替代物/氯仿后离心,样品分成水样层、中间层和有机层。RNA存在于水样层中。收集上面的的水样...