An RNA-binding protein is a type of protein that can bind to mRNA and regulate its translation by either stabilizing or destabilizing the transcript, thereby influencing the amount of protein produced from the
RNA结合蛋白(RNA-binding protein, RBP)和转录因子(transcription factor, TF)是两类不同的蛋白质,它们在调控基因表达中都扮演着重要的角色。 RNA结合蛋白是一类负责调控RNA后期加工、转运和降解的蛋白质,它们可以识别、结合特定的RNA序列或结构,并介导这些RNA的生物学功能。具体而言,RNA结合蛋白可以影响RNA的剪接、稳定...
et al. The RNA-binding protein FPA regulates flg22-triggered defense responses and transcription factor activity by alternative polyadenylation. Sci. Rep. 3, 2866; DOI:10.1038/srep02866 (2013). This work is licensed under a Creative Commons Attribution- NonCommercial-NoDerivs 3.0 Unported license....
近日,中国农业科学院植物保护研究所作物病原生物功能基因组研究创新团队在Nucleic Acids Research上发表了题为“APIP5 functions as a transcription factor and an RNA-binding protein to modulate cell death and immunity in rice”的研究论文,首次报道了植物bZIP类型转录因子APIP5具有结合DNA和RNA的双重活性,在转录...
Human antigen R (HuR) is an essential regulator of RNA metabolism, but its function in metabolism remains unclear. This study identifies HuR as a major repressor during adipogenesis. Knockdown and overexpression of HuR in primary adipocyte culture enhanc
(2002). A microbead-based system for identifying and characterizing RNA-protein interactions by flow cytometry. Mol. Cel. Proteomics 1(12):922-929. Gabrielsen OS et.al. (1989) Magnetic DNA affinity purification of yeast transcription factor t - a ...
Binding stoichiometry of an RNA aptamer and its transcription factor target. Anal. Biochem. 306, 290-297.Cassiday,L.A., Lebruska,L.L., Benson,L.M., Naylor,S., Owen,W.G. and Maher,L.J. (2002) Binding stoichiometry of an RNA aptamer and its transcription factor target. Anal. ...
Brodsky AS. and Silver A. (2002).A microbead-based system for identifying and characterizing RNA-protein interactions by flow cytometry.Mol.Cel.Proteomics 1(12):922-929. Gabrielsen OS et.al.(1989) Magnetic DNA affinity purification of yeast transcr...
Hfq是细菌中普遍存在的一种Sm-like RNA-binding protein,参与了细菌的生理适应度和发病机制,但其在体内结合机制尚不明确。采用 (CLIP-seq)方法探究得到Y. pestis中全基因组的hfq结合RNA。发现Y. pestis的Hfq结合80%以上为mRNA,而且Hfq结合非编码小RNA (sRNAs),Motif为AGAAUAA和GGGGAUUA。这些结果共同展示了高质...
与之相比,对照蛋白TBP(TATA box binding protein)和Rpb1 (RNA聚合酶 Pol II的关键蛋白)却不受RNA降解酶处理的影响。 这些结果说明,PRC2复合体不是直接和染色质结合的,而是间接地通过一道RNA的“桥梁”。当这个桥梁被降解的时候,PRC2就会从染色质上脱落。他们又通过另外一个实验支持了这一结论。当他们用RNA聚合...