The functions of RING-type E3 ubiquitin ligase family in response to abiotic and biotic stress LI Dayong (Institute of Biotechnology,Faculty of Agriculture,Zhejiang University,Hangzhou 310058) Abstract: The RING family is the largest type of E3 ubiquitin ligases which is specified by a RING domain...
Based on extensive structural analysis it was proposed that RING E3 ligases prime the E2~ubiquitin conjugate (E2~Ub) for catalysis by locking it into a closed conformation, where ubiquitin is folded back onto the E2 exposing the restrained thioester bond to attack by substrate nucleophile. However...
关键词:植物病理学;RING;E3泛素连接酶;抗逆;抗病中图分类号:S435.111ThefunctionsofRING-typeE3ubiquitinligasefamilyinresponsetoabioticandbioticstressLIDayong(InstituteofBiotechnology,FacultyofAgriculture,ZhejiangUniversity,Hangzhou310058)Abstract:TheRINGfamilyisthelargesttypeofE3ubiquitinligaseswhichisspecifiedbyaRING...
Attachment of ubiquitin (Ub) to proteins is one of the most abundant and versatile of all posttranslational modifications and affects outcomes in essentially all physiological processes. RING E3 ligases target E2 Ub-conjugating enzymes to the substr
研究发现,SmDDA1b是AtDDA1的同源蛋白,而AtDDA1蛋白是E3泛素连接酶(CRL4)的底物受体蛋白(Irigoyen et al., 2014)。底物受体蛋白(DDA1)和UPS系统中的支架蛋白CUL4(The cullin-RING ubiquitin-protein ligases)通过DNA损伤修复接合蛋白DDB1(Pang et al., 2019)结合到CRL4上。因此作者又通过Y2H实验、BiFC实验验证...
Ubiquitin ligase (E3)Ubiquitin-conjugating enzyme (E2)RING finger domain and RING finger-like ubiquitin ligases (E3s), such as U-box proteins, constitute the vast majority of known E3s. RING-type E3s function together with ubiquitin-conjugating enzymes (E2s) to mediate ubiquitination and are ...
The RING (Really Interesting New Gene) family is the largest type of E3 ubiquitin ligases. RING finger domains bind two zinc ions in a unique cross-brace arrangement through a defined motif of cysteine and histidine residues. This arrangement endows the RING domain with a globular conformation, ...
A RING Finger Protein is a type of E3 ubiquitin ligase that contains a RING finger domain, which is a folded domain consisting of seven cysteine residues and one histidine residue binding two zinc ions. This domain is critical for the transfer of ubiquitin to substrates or to other RING finge...
The E3 ubiquitin ligases normally provide the majority of the specificity and control the recognition of substrates as well as regulate the ubiquitination activity (Seirafi et al., 2015). Parkin comprises an N-terminal ubiquitin-like (UBL) domain and a C-terminal R1-in-between-ring-RING2 motif...
The two largest classes of RING proteins contain either no identifiable domain or a transmembrane domain. The presence of such a large and diverse number of RING domain-containing proteins that function as ubiquitin E3 ligases suggests that target-specific proteolysis by these E3 ligases is a ...