这一过程是由参与泛素激活酶(E1)、泛素结合酶(E2)和泛素连接酶(E3)的一系列酶反应介导的,这些酶反应在整个泛素化途径中会被ATP循环和激活,其中E3的作用是确定蛋白酶体中泛素化和随后降解的底物特异性。 人类基因组编码600多个E3泛素连接酶中,cullin-ring泛素连接...
8 Recently, cullin-RING E3 ubiquitin ligases (CRLs), the predominant E3 ubiquitin ligase family in eukaryotes, have been implicated in neuritogenesis. CRL is a multi-protein complex formed by the core scaffold protein cullin, which acts as a platform for the E2 enzyme and substrates with the ...
1.Potential of E3 Ubiquitin Ligases in CancerImmunity: Opportunities and Challenges. Cells. 2021 Dec; 10(12): 3309.
Regulation of cullin-RING E3 ubiquitin-ligases by neddylation and dimerization. Cell. Mol. Life Sci. 66:1924-1938.Merlet J, Burger J, Gomes JE, Pintard L. Regulation of cullin-RING E3 ubiquitin- ligases by neddylation and dimerization. Cellular and molecular life sciences : CMLS. 2009;66(...
相信随着研究的深入和临床的进展,这些问题都可以得到解决,靶向E3泛素连接酶将在肿瘤免疫治疗中展现出良好的应用前景。 参考文献: 1.Potential of E3 Ubiquitin Ligases in CancerImmunity: Opportunities and Challenges. Cells. 2021 Dec; 10(12): 3309.
目前用于靶向蛋白降解和稳定的效应蛋白分别是E3泛素连接酶(E3 ubiquitin-ligases)和去泛素酶(deubiquitinases)。在细胞中,E3泛素连接酶可通过将一种叫做泛素的小蛋白贴在靶蛋白上将其标记为缺陷或受损蛋白。之后,细胞内的蛋白粉碎机(即蛋白酶体)会处理掉被标记的靶蛋白。相反的,去泛素酶从蛋白质上去除泛素标签,从而...
植物Ｅ３泛素连接酶在调控激素响应、参与形态建成、抗病防御反应和非生物胁迫响应方面起着重要作用。ＲＩＮＧ（ＲｅａｌｌｙＩｎｔｅｒｅｓｔｉｎｇＮｅｗＧｅｎｅ１）家族是含环指结构（ＲＩＮＧｆｉｎｇｅｒｄｏｍａｉｎ）的Ｅ３泛素连接酶家族，一般定位于细胞核。含有跨膜结构域（ｔｒａｎｓｍｅｍｂｒａｎｅｄｏｍａｉｎ...
E3泛素连接酶NOT4包含环指结构域（RING finger）和RNA识别基序（RNA Recognition Motif，RRM），在蛋白质的水解和RNA生物学中发挥重要作用【4-6】。研究还发现，NOT4可作为酵母中乙酰化依赖的N-degron途径的E3连接酶【7】。尽管在酵母和哺乳动物中，NOT4在共翻译控制和调控mRNA与蛋白质稳定性的核心作用已被揭示，...
VHL & CRBN ligases both belong to family of cullin-RING E3 ubiquitinligases(CRLs). CRBN, substrate adaptor for the CRL4 E3 ubiquitin ligase, primary target of IMiD (POM,LEN, .etc). IMiDs enhance the binding of IKZF1&3 to CRBN,thus facilitating the degradation. ...
3. El Magraoui F, Bäumer BE, Platta HW, Baumann JS, Girzalsky W, Erdmann R. The RING-type ubiquitin ligases Pex2p, Pex10p and Pex12p form a heteromeric complex that displays enhanced activity in an ubiquitin conjugating enz...