The Cullin-RING ligases (CRLs) are the largest family of ubiquitin E3s activated by neddylation and regulated by the deneddylase COP9 signalosome (CSN). The inositol polyphosphate metabolites promote the formation of CRL–CSN complexes, but with unclear mechanism of action. Here, we provide struct...
E3泛素连接酶参与真核生物的多种生物学过程,许多E3泛素连接酶的活性可以用催化半胱氨酸反应的探针来检测,然而像cullin-RING泛素连接酶(cullin-RING ubiquitin ligases, CRLs)家族缺乏活性位点,而是连接底物和泛素携带酶。 CRLs是一个蛋...
Cullin-RING泛素连接酶(CRLs)是细胞中最重要的调控系统之一,特定CRL的突变会导致发育异常、高血压和各种癌症。CRL。并且现在已经有许多药企利用靶向蛋白降解这一技术,该技术是用小分子介导的E3泛素连接酶去泛素化蛋白底物,称为新底物(neo-substrate)。但是E3泛素连接酶如何靶向这些底物使其降解的机制仍不清楚。 近日,...
Hui ZhangFribourgh JL, Nguyen HC, Wolfe LS, Dewitt DC, Zhang W, Yu XF, Rhoades E, Xiong Y: Core binding factor beta plays a critical role by facilitating the assembly of the Vif-cullin 5 E3 ubiquitin ligase. J Virol 2014, 88:3309-3319....
Ubiquitin-dependent remodeling of the actin cytoskeleton drives cell fusion 2021, Developmental Cell Citation Excerpt : We focused on E3 ligases, as they control metazoan development and are mutated in distal and nemaline myopathies (Gupta and Beggs, 2014; Rape, 2018). Moreover, tissue-specific ...
Cullin-RING ubiquitin ligases (CRLs) comprise the largest known category of ubiquitin ligases. CRLs regulate an extensive number of dynamic cellular processes, including multiple aspects of the cell cycle, transcription, signal transduction, and development. CRLs are multisubunit complexes composed of a...
CuLlin参与泛素复合体(Ubiquitin Complex)的组成,为泛素连接酶(Ubiquitin Ligases E3)提供疏水蛋白骨架,几乎存在于所有真核生物。CuLlin与环蛋白(RING Proteins)形成枯环泛素连接酶复合体(CuLlin-RING Ubiquitin Ligases,CRLs),与泛素等在蛋白质的定位、代谢、功能、调节和降解中都起着十分重要的作用。参与细胞周期、增殖...
Cullin-RING ubiquitin ligases (CRLs) represent the largest family of E3 ubiquitin ligases that control most if not all cellular processes. In CUL3-based CRLs, the substrate specificity is conferred by the interaction with one of around 183 existing BTB proteins, implying a broad spectrum of ...
Cullin 2的基因位于人类10号染色体(10p11.21),分子量约87Kd,等电点6.46。Cullin参与泛素复合体(Ubiquitin Complex)的组成,为泛素连接酶(Ubiquitin Ligases E3)提供疏水蛋白骨架,几乎存在于所有真核生物。Cullin与环蛋白(RING Proteins)形成枯灵素环泛素连接酶复合体(Cullin-RING Ubiquitin Ligases,CRLs),与泛素等在...
蛋白活性,在多种生物学过程中发挥重要作用,目前在生殖系统中的研究日渐增多,本文总结了Culling家族各成员在配子发生与成熟、胚胎发育、生殖内分泌疾病及妇科肿瘤等生殖系统相关疾病中的作用.%Culling protein is a member of Cullin-Ring-based E3-ligases ( CRLs family) , which belong to E3 ubiquitin ligases. ...