The Cullin-RING ligases (CRLs) are the largest family of ubiquitin E3s activated by neddylation and regulated by the deneddylase COP9 signalosome (CSN). The inositol polyphosphate metabolites promote the formation of CRL–CSN complexes, but with unclear mechanism of action. Here, we provide struct...
E3 ubiquitin ligaseCullin 73-M diseaseGrowth signalingCRL7(Fbxw8) is an E3 ubiquitin ligase complex, containing cullin7 (CUL7) as a scaffold, the F-box protein Fbxw8 as a substrate receptor, the Skp1 adaptor, and the ROC1/Rbx1 RING finger protein for working with E2 enzyme to ...
The nomenclature for CRLs is only well established for CUL1-based SCF complexes. The naming of other CRL complexes is not settled, with various competing acronyms. For this review, we will refer to non-SCF CRL complexes by using the acronym "CRL" forcullinRING ubiquitinligase followed by the ...
PART.01 研究背景 VHL蛋白是Cullin RING E3连接酶的关键底物受体蛋白。它具有广泛的组织表达,并通过识别羟基化HIF-1α转录因子,充当缺氧应激反应的关键调节因子。 在常氧(高氧)条件下,HIF-1α在特定脯氨酸残基处被脯氨酰羟化酶 (PHD) 羟基化。这些羟脯氨酸标记可被 VHL 有效识别,VHL是E3连接酶CRL2的底物受体。
Kelch-like proteins, substrate adapters for the Cullin-3 (Cul3)-RING ligase (CRL3) complex, are emerging as critical regulators of striated muscle development and function, highlighting the importance of Cul3-mediated proteostasis in muscle function. To explore the role of Cul3-mediated ...
来调节蛋白活性,在多种生物学过程中发挥重要作用,目前在生殖系统中的研究日渐增多,本文总结了Culling家族各成员在配子发生与成熟、胚胎发育、生殖内分泌疾病及妇科肿瘤等生殖系统相关疾病中的作用.%Culling protein is a member of Cullin-Ring-based E3-ligases ( CRLs family) , which belong to E3 ubiquitin ...
在这里,我们利用小鼠模型发现,Cullin-RING E3泛素连接酶5 的关键成分 Cullin5(CUL5)上调,并与中性粒细胞数量增加和流感诱发的屋尘螨诱发的哮喘恶化有关。与此相反,CUL5 缺乏会通过增加IFN-β的产生来减轻中性粒细胞肺部炎症和哮喘的恶化。从机理上讲,胸腺基质淋巴细胞生成素刺激后,CUL5 与 O-GlcNAc 转移酶(OGT...
Among the three types of ubiquitination-related enzymes, E3 ubiquitin ligase is critical in determining substrate specificity. The importance of cullin-RING E3 ligase (CRL), a type of E3 ligase, has been emphasized during the recent decade due to its large number and its involvement in various ...
Anticancer targets, autophagy, cullins, CRL/SCF E3 ligase, MLN4924, NEDD8, neddylation, protein degradation, ubiquitin, UPSThe ubiquitin-proteasome system (UPS) promotes the timely degradation of short-lived proteins with key regulatory roles in a vast array of biological processes, such as cell ...
Among the E3 ubiquitin ligase families, the cullin-RING E3 ubiquitin ligase (CRL) family is the largest. Cullins are molecular scaffold proteins that recruit multiple subunits to form the CRL complex. We hypothesized that a CRL complex mediates the cGMP-dependent increase in NKCC2 ubiquitination ...