63]. In the absence of binding the CRL2 complex, VHL is degraded through a proteasome-dependent mechanism, presumably via the activity of another E3 [63]. Many other SRS proteins are also degraded through
Wirbelauer, C. et al. The F-box protein Skp2 is a ubiquitylation target of a Cul1-based core ubiquitin ligase complex: evidence for a role of Cul1 in the suppression of Skp2 expression in quiescent fibroblasts.EMBO J.19, 5362–5375 (2000). ...
Cullin 73-M diseaseGrowth signalingCRL7(Fbxw8) is an E3 ubiquitin ligase complex, containing cullin7 (CUL7) as a scaffold, the F-box protein Fbxw8 as a substrate receptor, the Skp1 adaptor, and the ROC1/Rbx1 RING finger protein for working with E2 enzyme to facilitate ubiquitin transfer...
Kelch-like proteins, substrate adapters for the Cullin-3 (Cul3)-RING ligase (CRL3) complex, are emerging as critical regulators of striated muscle development and function, highlighting the importance of Cul3-mediated proteostasis in muscle function. To explore the role of Cul3-mediated ...
suggest that CRL transition from the E2-bound active state to the CSN-bound sequestered state is critically assisted by an interfacial IP6 small molecule, whose metabolism may be coupled to CRL–CSN complex dynamics. 参考文献 1. Lin, H., Zhang, X., Liu, L., Fu, Q., Zang, C., Ding...
Quality control of protein complex composition 2022, Molecular Cell Citation Excerpt : When these enzymes bind a target, their cullin scaffold is modified with the ubiquitin-like NEDD8 and thereby protected from the adaptor exchange factor CAND1 (Baek et al., 2021; Liu et al., 2002; Zheng et...
both CUL7 and CUL9 contain a p53-binding domain or CPH (CUL7, PARC and HERC2-containing) domain and an APC10/DOC domain similar to that found in the APC/C ubiquitin ligase complex (Fig.1a). This structure contributes to the possibility that CRL7 and CRL9 may have functions redundant ...
In particular, both CUL3 and KLHL12, key members of the Cullin-RING (CRL) ubiquitin ligase family of proteins involved in procollagen transport from the ER to the Golgi, were highly up-regulated in CXCL12-, but repressed in TGFβ-, treated cells. Up-regulation of CUL3 and KLHL12 was ...
Mechanism of lysine 48-linked ubiquitin-chain synthesis by the cullin-RING ubiquitin-ligase complex SCF-Cdc34. Ubiquitin chains linked via lysine 48 (K48) of ubiquitin mediate recognition of ubiquitinated proteins by the proteasome. However, the mechanisms underlyin... MD Petroski,RJ Deshaies - 《...
remain unknown. Here we report the cryo-electron microscopy structure of a chemically trapped complex that represents the ubiquitylation intermediate, in which the neddylated CRL1β-TRCPpromotes the transfer of ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D to its recruited substrate, phosphory...