鱼类特有的Ring型E3泛素连接酶MARCH5A基因在刺激隐核虫感染下的表达分析(Expression analysis of fish-specific Ring type E3 ubiquitin ligase MARCH5A gene with Cryptocaryon irritans induction)为探究大黄鱼5的免疫作用,实验采用反转录PCR确认了该基因的cDNA序列。该序列全长1045 bp,包含1个长度为867 bp的开放阅读...
A Large Complement of the Predicted Arabidopsis ARM Repeat Proteins Are Members of the U-Box E3 Ubiquitin Ligase Family The Arabidopsis genome was searched to identify predicted proteins containing armadillo (ARM) repeats, a motif known to mediate protein-protein interaction... Mudgil,Y. - 《Plant...
As indicated in Figure 2E, one of the RNF13 RING finger domain mutants, C258A/H260A, completely lost its E3 ligase activity while the other mutant W270A maintained little E3 ligase activity. In summary, these data confirm that RNF13 is a bona fide RING-type E3 ubiquitin ligase. RNF13 ...
Two pathways in ubiquitylation are linked, as the RING domain E3 ligase Ubr1, from the N-end rule pathway, is found to bind and cooperate with the HECT domain E3 Ufd4 from the ubiquitin-fusion degradation pathway. Substrates of the N-end rule pathway are
(2007) SDIR1 is a RING finger E3 ligase that positively regulates stress-responsive abscisic acid signaling in Arabidopsis. The Plant Cell Online 19: 1912–1929. doi: 10.1105/tpc.106.048488 [12] Kulye M, Liu H, Zhang Y, Zeng H, Yang X, et al. (2012) Hrip1, a novel protein ...
Here, we cloned a novel RING-H2 type E3 ubiquitin ligase gene, named IbATL38, from sweetpotato cultivar Lushu 3. This gene was highly expressed in the leaves of sweetpotato and strongly induced by NaCl and abscisic acid (ABA). This IbATL38 was localized to nucleus and plasm membrane and ...
Thus, Rma1 represents a novel, membrane-bound type of ubiquitin ligase E3, which probably functions with the Ubc4/5 subfamily of E2. The MBP moiety but not Rma1 itself is ubiquitinated in the auto-ubiquitination reaction of MBP-Rma1. Free MBP in solution is not a substrate of Rma1. ...
The E3 ubiquitin ligase activity of mammalian hVps18 is also dependent on the presence of this domain [55]. The CORVET complex is most likely required for the fusion of Golgi-derived vesicles with the endosome [56,57]. Show abstract Disruption of lysosome function promotes tumor growth and ...
Ubiquitin ligase (E3)Ubiquitin-conjugating enzyme (E2)RING finger domain and RING finger-like ubiquitin ligases (E3s), such as U-box proteins, constitute the vast majority of known E3s. RING-type E3s function together with ubiquitin-conjugating enzymes (E2s) to mediate ubiquitination and are ...
Ubiquitin ligase (E3Ubiquitin-conjugating enzyme (E2Protein degradationCatalysis•RING-type E3s constitute a large and diverse class of ubiquitin ligases.•RING-type E3s exist as monomers, dimers, and in complex multi-subunit assemblies.•RING-type E3s recognize substrates by a variety of ...