近日,华中农业大学姜道宏团队在Plant Physiology在线发表了题为“Protist ubiquitin ligase effector PbE3-2 targets cysteine protease RD21A to impede plant immunity”的研究论文,揭示了根肿菌分泌E3泛素连接酶靶向降解拟南芥免疫相关半胱氨酸蛋白酶RD21A以抑制寄主抗性的分子机制。 研究人员首先从根肿菌ZJ-1菌株基因...
3.1PbE3-2是一种RING型E3泛素连接酶 通过使用Blast2GO系统研究甘蓝型油菜ZJ-1的基因组,预测有60个基因编码推定的E3泛素连接酶。我们假设这些E3泛素连接酶中的一些被分泌到宿主细胞中,并有助于成功感染。60个预测的E3泛素连接酶中有5个具有信号肽,分别命名为PbE3-1、PbE3-2、PbE3-3、PbE3-4和PbE3-5...
two ubiquitin E3 ligases containing plant U-box (UPB) domains, PUB12/13, are phosphorylated by BAK1, which is required for FLS2-PUB12/13 association. Then, PUB12/13 polyubiquitinate FLS2 and promote
The occurrence of premature stop codons might cause the formation of truncated, non-functional MpNOP1 proteins not comprising the crucial U-box of the E3 ubiquitin-protein ligase. Also, aberrant mRNAs might be eliminated via the non-sense mediated mRNA decay mechanism which prevents the synthesis ...
U-boxPlant U-box armadillo repeat (PUB-ARM) proteins represent a type of E3 ubiquitin ligase. PUB-ARM proteins have various functions in plant development, plant defence and plant stress responses. The so far uncharacterised PUB-ARM proteins AtPUB18 and AtPUB19 are highly homologous to each ...
例如,ABA受体PYR/PYL/RCAR家族的几个成员可以被不同的E3泛素连接酶特异性识别,并通过泛素-26S蛋白酶体系统(Ubiquitin-26S proteasome system,UPS)降解;负调控ABA信号的PP2C磷酸酶ABI1可以被PUB12/PUB13(一种U-box E3 ligases)泛素化并被蛋白酶体降解,进而促进SnRK2激酶及其下游转录因子的活化【3】。此外,当ABA...
32. Yang CW, Gonzalez-Lamothe R, Ewan RA, Rowland O, Yoshioka H, Shenton M, Ye H, O’Donnell E, Jones JD, Sadanandom A: The E3 ubiquitin ligase activity of arabidopsis PLANT U-BOX17 and its functional tobacco homolog ACRE276 are required for cell death and defense. Plant Cell ...
In this study, we show that two U-box type E3 ubiquitin ligases, PUB25 and PUB26, interact with the negative regulator of cold signaling, MYB15, to enhance cold stress responses in Arabidopsis. Biochemical and genetic analyses showed that PUB25 and PUB26 act upstream of the CBF pathway to...
E3 Ubiquitin Ligase CHIP and NBR1-Mediated Selective Autophagy Protect Additively against Proteotoxicity in Plant Stress Responses (2014). E3 Ubiquitin Ligase CHIP and NBR1-mediated selective autophagy protect additively against proteotoxicity in plant stress responses. PLoS Genet. 10:e... P P - 《...
Here, we report that an ABA-responsive U-box E3 ubiquitin ligase, PLANT U-BOX 35 (PUB35), physically interacts with AFP1 and ABI5. PUB35 directly ubiquitinated ABI5 in a bacterially reconstituted ubiquitination system and promoted ABI5 protein degradation in vivo. ABI5 degradation was enhanced...