Cytosolic ligase-based degrader platforms exploit a limited number of E3 ubiquitin ligases, partly owing to the challenge of obtaining high-affinity ligands23. We sought to explore whether PROTABs could utilize a broader range of cell-surface E3 ubiquitin ligases. Of the 449 known E3 ubiquitin li...
Ring-finger protein 5 (RNF5) is an E3 ubiquitin ligase which is expressed in a variety of human tissues. RNF5 is involved in the regulation of endoplasmic reticulum stress, inflammation, and innate immunity and plays an important role in the occurrence and development of various tumors. However...
In conclusion, given that the quiescence state is the basis for self-renewal and multilineage differentiation, the effect of E3 ubiquitin ligase on this state is likely to cause alterations in cell cycle (Fig.3), thus triggering different degrees of alterations in the ability to self-renew and ...
We show that Polɩ is not a direct substrate of RNF2 E3 ubiquitin ligase activity. Nonetheless, the activity, but not the ability of RNF2 to interact with Polɩ, is important for the maintenance of Polɩ cellular levels and prevents its destabilization. Furthermore, we report that the...
Hence, we add TRIB2 to the substrate list of SCFβ-TRCP and the finding may be helpful in the treatment of TRIB2 dependent liver cancer.YongxiaQiaoYueZhangJiayiWangSDOSBiochemical & Biophysical Research CommunicationsQiao, Y.; Zhang, Y.; Wang, J. Ubiquitin E3 ligase SCF(β-TRCP) regulates...
Using Proximity-based Proteomics to Identify Interacting Partners and Substrates for the Listerin Ubiquitin Ligase Author(s): Zuzow, Nathaniel David | Advisor(s): Bennett, Eric J | Abstract: The ribosomal quality control complex (RQC) is a multiprotein complex which senses, modifies, and extracts...
and other physiological processes. They are closely associated with the occurrence and development of neurodegenerative diseases. DUB, deubiquitinating enzyme; E1, E1 ubiquitin-activating enzyme; E2, E2 ubiquitin-conjugating enzyme; E3, E3 ubiquitin ligase; Ub, ubiquitin. This schematic is created with...
Recent studies demonstrated that depletion of SAG (Sensitive to Apoptosis Gene), an E3 ubiquitin ligase, blocked inflammatory responses generated by macrophages and dendritic cells in response to LPS in cell culture settings. However, the in vivo role of Sag on modulation of macrophages and ...
The third enzyme (E3) is the ubiquitin ligase acting as a scaffold protein that interacts with the E2 enzyme and transfers ubiquitin to the target protein.2,3 This process is reversible through the action of deubiquitinases (DUBs) that remove ubiquitin chains linked to the target protein.4 ...
Table 1 List of the characteristics of the identified bands. Full size table We analyzed the type of ubiquitin chain formed on Nur77 as a result of Trim13 activity. For this, we used a set of ubiquitin mutants in which all except one Lys residue was replaced by Arg. The ubiquitination ...