CUL5-ARIH2 E3-E3 ubiquitin ligase structure reveals cullin-specific NEDD8 activationAn emerging mechanism of ubiquitylation involves partnering of two distinct E3 ligases. In the best-characterized E3-E3 pathways, ARIH-family RING-between-RING (RBR) E3s ligate ubiquitin to substrates of neddylated ...
2024年2月28日,复旦大学张英梅、葛均波及中国科学院分子细胞科学卓越创新中心胡荣贵共同通讯在Cell Discovery(IF=34)在线发表题为“The E3 ubiquitin ligase MARCH2 protects against myocardial ischemia-reperfusion injury through inhibiting pyro...
近日,北京大学基础医学院系统生物医学研究所尹玉新教授团队在The EMBO Journal上发表了题为“Structural Insights into the Ubiquitylation Strategy of the Oligomeric CRL2FEM1B E3 Ubiquitin Ligase”的文章,报道了多聚形态E3泛素连接酶CRL2FEM1B在NEDD8修饰激活前,NEDD8修饰激活后与小分子量底物BEX2(约15kDa)结合...
Ubiquitin ligase (e3) is the key factor of the eukaryotic protein selective degradation in vivo system (ups) to regulate a variety of physiological and biochemical reactions in eukaryotic cells. 翻译结果2复制译文编辑译文朗读译文返回顶部 With the aggravation day by day of the energy crisis of the...
mutation of C431 eliminates Parkin-catalysed degradation of mitochondria, and capture of an ubiquitin oxyester confirms C431 as Parkin’s cellular active site. Our data confirm that Parkin is a RING/HECT hybrid, and provide the first crystal structure of an RING-between-RING E3 ligase at atomic...
Structure of an E6AP-UbcH7 complex: insights into ubiquitination by the E2-E3 enzyme cascade. Science 286, 1321–1326 (1999). CAS PubMed Google Scholar Verdecia, M. A. et al. Conformational flexibility underlies ubiquitin ligation mediated by the WWP1 HECT domain E3 ligase. Mol. Cell 11...
E3 is most directly responsible for substrate recognization. It contains three members, including HECT domain E3, RING domain E3, and U-box domain E3. Here, we summarize the classification, the structure and the mechanism of substrate recognization of ubiquitin-protein ligase enzyme (E3).关键词:...
在该过程中,起决定性作用的E3泛素化连接酶能介导泛素蛋白连接到靶蛋白上,因此针对E3进行PROTAC药物的弹头及分子胶降解剂(Molecular glue degrader, MGD)正逐渐被人们所关注和深入探究。最近,来自阿斯利康的两位研究者联合在JMC上发表综述文章,总结近年来基于结构的先导化合物发现策略(Fragment-based lead discovery, FB...
E3 ubiquitin ligases often reside in multiple subcellular compartments in neurons, and thus defining the precise compartment relevant for a specific biological function may not be trivial. Targeting an E3 ligase to a specific subcellular locale in structure–function analyses using specific peptide motifs...
样本:CLIA Kit for Human E3 ubiquitin-protein ligase AMFR 检测方法:夹心法 应用:夹心法 检测限:血清、血浆、组织匀浆、细胞培养物上清或其它相关液体 货号:FS-C6349 是否进口:是 标本要求: 1.血清: 室温血液自然凝固10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再...