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Single-cell epigenome sequencing techniques have recently been developed. However, the combination of different layers of epigenome sequencing in an individual cell has not yet been achieved. Here, we developed a single-cell multi-omics sequencing techno
Total RNA was treated with TURBO DNA-free™ (Ambion, Huntingdon, UK) for removal of contaminating DNA and first strand cDNA synthesis was conducted on 0.5 μg total RNA using SuperScript™-II Rnase H- Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA). Assays for the genes included in...
Loneliness, a complex socio-emotional trait is a strong predictor of mental illness. Loneliness has been associated with larger GMV of the dlPFC and smaller volume of amygdala, anterior hippocampus, posterior para-hippocampus, and cerebellum [59,60,61], interestingly in the left hemisphere for all...
Cite this paper Williams, R. and Al-Hmoud, R. (2015) Virtual Water on the Southern High Plains of Texas: The Case of a Nonrenewable Blue Water Resource.Natural Resources,6, 27-36. doi:10.4236/nr.2015.61004. References [1]Hoekstra, A.Y. and Chapagain, A.K. (2007) Water Footprints ...
IAV is negative-strand RNA virus and belongs to the family Orthomyxoviridae. It is composed of eight gene segments that encode at least 17 different kinds of protein [5,6,7]. Hemagglutinin (HA) is main envelope glycoprotein and can be cleaved into the HA1 and HA2 subunit. Continual mutation...
We have previously developed an OI approach for early detection of oral cancer that targets the DNA repair enzyme PARP1. PARP1 plays a central role in initiating the DNA damage repair response (DDR), especially for single-strand breaks [22,23]. PARP1 expression is strongly increased in many...
Hum. Brain Mapp. 31, 904–916. Kramer, A.F., Erickson, K.I., 2007. Capitalizing on cortical plasticity: influence of physical activity on cognition and brain function. Trends Cogn. Sci. 11, 342–348. Kretschmann, H.J., Kammradt, G., Krauthausen, I., Sauer, B., Wi...
(MAPQ) ≥ 10 were counted for overlap with genomic features using featureCounts of subread version 1.5.0-p137, without strand information. Autosomal feature counts with > 10 mean counts across samples were then normalized and compared between-group using DESeq2 version 1.12.338. Since gender is...
Bone defects caused by diseases and trauma are usually accompanied by inflammation, and the implantation of biomaterials as a common repair method has also been found to cause inflammatory reactions, which affect bone metabolism and new bone formation. T