The application of rapidly growing CRISPR toolboxes and methods has great potential to transform biomedical research. Here, we provide a snapshot of up-to-date CRISPR toolboxes, then critically discuss the promises and hurdles associated with CRISPR-based nuclear genome editing, epigenome editing, an...
Huntington’s disease (HD) is an autosomal dominantly-inherited neurodegenerative disease, which is caused by CAG trinucleotide expansion in exon 1 of the Huntingtin (HTT) gene. Although HD is a rare disease, its monogenic nature makes it an ideal model in which to understand pathogenic mechanisms...
Uterine leiomyosarcoma (uLMS) is a rare and aggressive gynaecological malignancy, with individuals with advanced uLMS having a five-year survival of < 10%. Mutations in the homologous recombination (HR) DNA repair pathway have been observed in
Strand V . Are COX-2 inhibitors preferable to non-selective non-steroidal anti-inflammatory drugs in patients with risk of cardiovascular events taking low-dose aspirin? Lancet 2007 ; 370 : 2138 – 2151 . VI Article CAS PubMed Google Scholar Arellano FM, Yood MU, Wentworth CE, Oliveria SA...
6 Adelphi Terrace, Strand, where I first became acquainted with Mr. John Hutt their secretary and afterwards Governor of Western Australia. (See the account of South Austa in the (London) Morning Herald of the 1st March 1837.) I could not find the article Philip referred to in the ...
An alkylation-tolerant, mutator human cell line is deficient in strand-specific mismatch repair. Proc Natl Acad Sci USA 1993; 90:6424–6428. Article CAS PubMed PubMed Central Google Scholar Koi M, Umar A, Chauhan DP, et al. Human chromosome 3 corrects mismatch repair deficiency and ...
(Thermo Scientific, Massachusetts, USA). Commercial DNA complementary strand formation kits (TOPScript™, Enzynomics Co. Ltd., Daejeon, Korea) were utilized for the synthesis of first-strand cDNAsviatotal RNA reverse transcription. Subsequently, gene expression qRT-PCR amplification reactions were ...
6 Adelphi Terrace, Strand, where I first became acquainted with Mr. John Hutt their secretary and afterwards Governor of Western Australia. (See the account of South Austa in the (London) Morning Herald of the 1st March 1837.) I could not find the article Philip referred to in the ...
In addition, the reduction of SIgA or pIgR levels is also related to the severity of COPD. A study found, the level of pIgR in the bronchial epithelium of severe COPD patients was lower than that in the control group, and the pIgR and IgA transport in the bronchial epithelial cell of se...
Endothelial cells (ECs) are widely used as donor cells in tissue engineering, organoid vascularization, and in vitro microvascular model development. ECs are invaluable tools for disease modeling and drug screening in fundamental research. When treating