Uterine leiomyosarcoma (uLMS) is a rare and aggressive gynaecological malignancy, with individuals with advanced uLMS having a five-year survival of < 10%. Mutations in the homologous recombination (HR) DNA repair pathway have been observed in
Governing AI-Driven Health Research: Are IRBs Up to the Task? Ethics Hum Res. 2021;43:35–42. Article Google Scholar Fullerton SM, Lee SS-J. Secondary uses and the governance of de-identified data: lessons from the human genome diversity panel. BMC Med Ethics. 2011;12:16. Article ...
This process gives rise to two products during plus(+) strand synthesis: partially circular rcDNA and double-stranded linear DNA (dslDNA). Rather than being encapsulated and secreted as virions, dslDNA has the potential to re-enter the nucleus and integrate into the human genome [5] (Fig. ...
Ovarian cancer (OC) remains the primary cause of mortality among gynecological malignancies, and the identification of reliable molecular biomarkers to prognosticate OC outcomes is yet to be achieved. The gene palmitoyl protein thioesterase 2 (PPT2), whi
Single-cell epigenome sequencing techniques have recently been developed. However, the combination of different layers of epigenome sequencing in an individual cell has not yet been achieved. Here, we developed a single-cell multi-omics sequencing techno
Accumulating evidence has suggested that sleep disturbances and disorders are common in patients who undergo knee arthroplasty. Revision surgery represents one of the most catastrophic outcomes of knee arthroplasty. However, it remains unclear whether sl
The total RNA of 12 samples were isolated by the TRIzol Reagent following the manufacturer's guidance (Ambion, USA). Next, total RNA were inversely transcribed into cDNA utilizing the SweScript-First-strand-cDNA-synthesis-kit (Servicebio, China), according to the manufacturer's protocol. QPCR ...
Osteofibrous dysplasia (OFD) is a rare, benign, self-limited bone disorder with a relatively low incidence, accounting for approximately 0.2% of all primary bone tumors. It was frequently found intra-cortical of the mid-shaft of the tibia. OFD can also o
The human brain is well known to exhibit plasticity, an intrinsic ability to reorganise its structure and function throughout the lifespan [4]. This allows the brain to adapt to changes in the external environment or internal milieu. With respect to external environmental influences, like the ex...
(Thermo Scientific, Massachusetts, USA). Commercial DNA complementary strand formation kits (TOPScript™, Enzynomics Co. Ltd., Daejeon, Korea) were utilized for the synthesis of first-strand cDNAsviatotal RNA reverse transcription. Subsequently, gene expression qRT-PCR amplification reactions were ...