multiplex PCRHPLCReal-time PCRaflatoxinsmycotoxinsmaize grainsmycotoxigenic fungifumonisinsThis study aimed to identify important mycotoxigenic fungi and accurate detection of mycotoxin in stored maize grains using molecular methods. The current study also optimised the real-time PCR (RT-PCR) assay. The...
It was demonstrated recently that the TaqMan probes which have been long used in Real Time PCR may also be utilized in DMA (Huang et al., 2011) [4]. After optimization of this method Botezatu et al. [1], it was used for multiplex scanning PIK3CA hotspot mutations in formalin-fixed ...
Besides the mutual competition of the individual PCR reactions included in a multiplex PCR assay, their specific read-out displays a limiting factor for the total number of PCR reactions that can be multiplexed. In this study, two PCR systems with different read-out approaches are compared, ...
Multiplex, real-time, one-step RT-PCR enables simultaneous quantification of several transcripts in one reaction vessel. This saves time and conserves precious sample material when analyzing the expression of reference and target genes in a particular sample. However, intensive PCR optimization is often...
Real-time RT-PCR是qPCR和RT-PCR的组合,其中的“RT”是Reverse transcription(逆转录)的意思,所以RT-qPCR就是结合了荧光定量技术的逆转录PCR,即以mRNA或总RNA为模板,先反转录得到cDNA,再以cDNA为模板,通过荧光定量PCR进行定量检测分析。因为RT-PCR只可以定性,但不能进行定量分析。与RT-PCR一样,RT-qPCR定量分析...
He has taught basic biology, microbiology, and anatomy and physiology at a university level for 5 years.Cite this lesson This lesson will cover the variation of the molecular technique, polymerase chain reaction (PCR). We will cover the basic concepts of a real-time, nested, and multiplex ...
(88.2%) typeable samples for Realtime-PCR and 19/34 (55.9%) for MS-PCR, p=0.007, 95%CL 0.04–0.66); the presence of multiple pneumococcal serotypes in nasopharyngeal swabs was found more frequently by Realtime PCR (19/30; 63.3%) than by Multiplex-sequential PCR (3/19; 15.8%; p=...
Multiple pathogens were detected in 29 (24%) of those samples. The tandem multiplex real-time PCR was an efficient method for the rapid detection of multiple pathogens. 展开 关键词: mixed infections respiratory pathogens tandem multiplex real-time PCR ...
Efficiency of Real-Time PCR (qPCR) What is multiplex qPCR? Support Real-Time PCR Support Center Real-Time PCR Troubleshooting Tool Real-Time PCR Data Analysis Real-Time PCR Training Series – live courses and web-based training Real-Time PCR Resource Library ...
Furthermore, the multiplex detection limit for pan-AIV and H5-AIV RRT-PCR was 5 copies per reaction. When this multiplex method was applied in the detection of experimental and live poultry market samples, the detection rates of pan-AIV and H5 AIV in RRT-PCR were both higher than the ...