Real time PCR, Multiplex PCR, Color multiplexing, Tm multiplexing, Rapid cycle PCR, Simple hybridization probes, Color compensation, Melting curve analysisThe ability to multiplex PCR by probe color and melting temperature (Tm) greatly expands the power of real-time analysis. Simple hybridization ...
Multiplex Real-Time PCR Assays for Rapid Detection of Hand, Foot and Mouth Disease(HFMD) Real-Time PCR Kit for Detection of Human Enterovirus Multiplex Real-Time PCR Diagnostic Kit for Rapid Detection of 6 Respiratory Viruses Instrument >> HFMD is a widespread infectious disease globally, large...
The primer and probe sets described for the different genes can be used either in single or in multiplex PCR assays because the individual probes were designed with different fluorochromes. The assays were found to be both sensitive and specific; the lower limit of the detection was 10–100 ...
Multiplex real-time PCR assays for the detection of group C rotavirus, astrovirus, and Subgenus F adenovirus in stool specimens. J. Virol. Methods 2013, 191, 141-147. [CrossRef] [PubMed]Kohji Mori,Yukinao Hayashi,Tetsuya Akiba,Miyuki Nagano,Tatsuya Tanaka,Mitsugu Hosaka,Akiko Nakama,Akemi ...
Real‐time PCR tests successfully detected Heterobasidion species from 36 fruiting bodies taken from the forest, as well as from artificially inoculated or naturally infected wood samples. The multiplex real‐time PCR assays developed in this study could have practical applications both in forest ...
19.WittwerCT, Herrmann MG, Gundry CN, Elenitoba-Johnson KS. Real-time multiplex PCR assays.Methods2001;25:430–42.10.1006/meth.2001.1265Search in Google ScholarPubMed 20.WilkeningS, Bader A. Quantitative real-time polymerase chain reaction: methodical analysis and mathematical model.J Biomol Techn...
-based real-time multiplex PCR assay for detection of toxin genes alpha (), beta (), iota (), epsilon (), beta2 () and enterotoxin () of , , , , and were detected in 68 (28.2%), 6 (2.5%), 6 (2.5%), 4 (1.6%), 164 (68%) and 11 (4.5%) of 241 PCR positive samples...
Having high-quality assays, molecular controls, and automated workflow solutions are critical in today’s high-capacity, fast-paced laboratory environments. Seegene USA has manufactured assays with advanced multiplex PCR technology since 2000. The company’s user-friendly workfl...
(PCR) doesn't. Regarding this, innovation combining high sensitivity and specificity, low cost, operational simplicity and practicability is in urgent need. Daan Gene will continue working on developing promising alternative assays to push the progress of the approach...
Comparison of singleplex and multiplex gene expression assays. (A) EGFR assay, FAM dye; (B) BRCA1 assay, VIC dye; (C) ESR1 assay, JUN dye; (D) RNase P assay, ABY dye. Amplification was performed on the QuantStudio 7 Real-Time PCR System using TaqMan Multiplex Master Mix. The figure...