Smart-Plexer: a breakthrough workflow for hybrid development of multiplex PCR assays Article Open access 09 September 2023 Introduction The advance of high throughput sequencing has uncovered a large number of biomedically relevant DNA sequences, from driver mutations in cancer to new bacterial/viral...
Demonstration of array-based analysis for highly multiplexed PCR assays application to detection of IGH@-BCL2 translocations in FFPE follicular lymphoma specimens. J Mol Diagn. 2011;13(3):252-262.Spence JM, Rothberg PG, Wang N, et al.Demonstration of ar- ray-based analysis for highly ...
Xiao, X.-let al. Simultaneous Detection of Salmonella, Listeria monocytogenes, and Staphylococcus aureus by Multiplex Real-Time PCR Assays Using High-Resolution Melting.Food Anal. Methods7, 1960–1972 (2014). ArticleGoogle Scholar Teethaisong, Y.et al. Phenotypic detection of AmpC beta-lactamases...
We designed two triplex droplet digital PCR assays, each with 2 unique targets and 1 in common, and normalize the results to PCR-based T cell counts. Both HIV assays are specific, sensitive, and reproducible. Together, they estimate the number of proviruses containing all five primer-probe ...
Multiplex PCRVirus resistanceGenetically modifiedPapayaSquashTwo separate multiplex PCR assays were developed for the detection of transgenic papaya and transgenic ... E Wall,T Lawrence,M Green,... - 《European Food Research & Technology》 被引量: 44发表: 2004年 A detection ofrecombinant DNAs from...
Our purpose was to evaluate the utility of the system in highly multiplexed gene expression assays. The method was highly sensitive and provided linear responses for the small tumor cell numbers expected in the blood of many cancer patients. The hands-on time for HyCEAD amplification is less tha...
aureus MRSA, and plaque assays were used for host range determination on Luria-Bertani (LB) media. The phage morphology of the isolated phage was determined by transmission electron microscopy. The phage’s whole genome sequencing was identified. Results A total of 25 isolates of Staphylococci ...
Multiplex IDentifier IMGT: International ImMunoGeneTics information system PCR: Polymerase Chain Reaction SSP: Sequence-Specific Primer assay SSO: Sequence-Specific Oligonucleotide assay SNP: Single Nucleotide Polymorphism SPRI: Solid Phase Reversible Immobilization emPCR: emulsion PCR HIV: Human Imm...
The PCR program for the multiplex editing sample involved an additional extension step at 72 °C for 1 m. The final PCR product was purified using the Monarch PCR and DNA cleanup kit (NEB, USA). T7E1 assay The purified 200 ng PCR product was denatured at 95 °C for 5 m,...
multiplexed biomarker assays have been developed. Such strategies have been attempted in several areas, including Alzheimer’s disease6,7, amyotrophic lateral sclerosis8, cardiovascular disease9,10,11, chronic obstructive pulmonary disease12, infection13and cancers14,15,16,17. Many of the methodologies...