Multiplex real-time PCRToluidine blue OAs observed from the results, multiplex real-time PCR demonstrated high sensitivity and specificity when compared to the culture technique. Therefore, it can prove to be a highly sensitive technique to detect the endodontic infections microflora....
It was demonstrated recently that the TaqMan probes which have been long used in Real Time PCR may also be utilized in DMA (Huang et al., 2011) [4]. After optimization of this method Botezatu et al. [1], it was used for multiplex scanning PIK3CA hotspot mutations in formalin-fixed ...
In this study, two PCR systems with different read-out approaches are compared, using a pentaplex PCR assay for the detection of highly pathogenic agents. A pentaplex assay was used since five represents the current limit of real-time PCR multiplexing capacity due to the low resolution of ...
(88.2%) typeable samples for Realtime-PCR and 19/34 (55.9%) for MS-PCR, p=0.007, 95%CL 0.04–0.66); the presence of multiple pneumococcal serotypes in nasopharyngeal swabs was found more frequently by Realtime PCR (19/30; 63.3%) than by Multiplex-sequential PCR (3/19; 15.8%; p=...
He has taught basic biology, microbiology, and anatomy and physiology at a university level for 5 years.Cite this lesson This lesson will cover the variation of the molecular technique, polymerase chain reaction (PCR). We will cover the basic concepts of a real-time, nested, and multiplex ...
Multiplex, real-time, one-step RT-PCR enables simultaneous quantification of several transcripts in one reaction vessel. This saves time and conserves precious sample material when analyzing the expression of reference and target genes in a particular sample. However, intensive PCR optimization is often...
以下是我们可能会接触或认识到的19种PCR类型。 1、实时荧光定量PCR(Quantitative Real-time PCR, qPCR) Real-time-PCR 和 qPCR 是一码事。实时荧光定量PCR,也叫Real-Time PCR,即二代PCR,是指在PCR扩增反应体系中加入荧光染料或者荧光基团,在整个PCR过程中通过收集荧光信号实时监测每一个循环中扩增产物量的变化,...
An economical tandem multiplex real-time PCR technique for the detection of a comprehensive range of respiratory pathogens. Viruses 2009;1:42-56... G Chidlow,G Harnett,G Shellam,... - 《Viruses》 被引量: 107发表: 2009年 An Economical Tandem Multiplex Real-Time PCR Technique for the Detecti...
What is multiplex qPCR? Support Real-Time PCR Support Center Real-Time PCR Troubleshooting Tool Real-Time PCR Data Analysis Real-Time PCR Training Series – live courses and web-based training Real-Time PCR Resource Library Innovative tools and educational resources that help advance th...
Conclusions: This real-time multiplex PCR technique can be carried out in a single tube and can differentiate between the three polymorphic sites of the two genes associated with Alzheimer's disease. Clin Chem Lab Med 2007;45:346–50. 展开 ...