Multiplex PCR assay for simultaneous detection and differentiation of Mycobacterium tuberculosis, Mycobacterium avium complexes and other mycobacterial species directly from clinical specimens. J Appl Microbiol. 2009;107:425–35. Article CAS PubMed Google Scholar Becton, Dickinson USA, BACTEC. MGIT 960 ...
Q. I have been asked to return money because of the use of multiplex polymerase chain reaction (PCR) urine cultures for patients. For each patient, I believe the PCR test ordered was medically necessary, and there were no rules at the time regarding the use of PCR. Can you help? A.We...
There is provided a method for designing primers for multiplex PCR, for assigning priorities to candidate amplification regions on same chromosomal DNA and designing primers for PCR amplifying the candidate amplification regions according to the priorities, the method having a feature in a method for...
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When calculating melting temperatures using online tools, such as the IDT SciTools™ OligoAnalyzer™ Tool and PrimerQuest™ Tool, be sure to enter the appropriate conditions from your PCR or qPCR experiment. Commonly used parameters are: 50 mM K+, 3 mM Mg2+, and 0.8 mM dNTPs; however...
PCR (QF-PCR), SNP genotyping, detection of aneuploidy, and more. While sequencing technologies also enable these applications, researchers choose fragment analysis because it has a faster turnaround time, higher sensitivity and is higher resolution. It is also more...
In the past few years, newer types of stool tests have been developed and are being used with greater frequency – these are called multiplex polymerase chain reaction (PCR) gastrointestinal syndrome panels. It is a complicated name, but it means that a single test can be used to test for ...
If your PCR machine needs to run overnight, try altering the settings so it holds at 10oC rather than 4oC. 6. Check your freezers Freezers are essential for long-term storage, but they require a huge amount of energy to power. To keep them efficient check the rubber seals are working ...
™ a comprehensive cell line authentication service that combines interspecies and intraspecies testing to verify the identity of human cell lines. Testing for interspecies contamination is performed by multiplex PCR and identifies DNA from the species of cell lines that are most commonly used in ...
The Philadelphia chromosome is present in approximately 20% to 30% of adults with acute lymphoblastic leukemia (ALL). The poor prognosis of this relatively... AK Fielding 被引量: 0发表: 2013年 An efficient multiplex genotyping approach for detecting the major worldwide human Y-chromosome haplogrou...