应用PCR扩增牛SRY序列进行奶牛胚胎性别鉴定 摘要: 曾溢滔,张美兰,陈美珏,... - 《中国科学》 被引量: 202发表: 1993年 Micronanipulation of bovine embryos for sex determination All sectors of the cattle industry derive economic benefit from the ability to determine the sex of calves before pregnancy...
a本文主要介绍多重PCR、等位基因特异PCR、缺口PCR,现在在遗传疾病诊断方面的具体应用,并针对其原理和诊断过程中的缺陷提出建议。最后,初步估计它们的发展前景和发展方向。 This article main introduction multiple PCR, allele special PCR, gap PCR, diagnose the aspect now at the heredity disease the concrete ...
PCR, sometimes known as “molecular photocopying’, is frequently hailed as one of the most significant scientific developments in molecular biology. It revolutionized the study of DNA to the point where its inventor, Kary B. Mullis was awarded the Chemistry Nobel Prize in 1993. Without PCR ampli...
The assessment of yield and quality of DNA after isolation by HTSO method was done using NanoDrop-8000. The DNA isolated by the HTSO was used in the genotyping of multiple mutations, R402W and W225X in the Glutaryl-CoA Dehydrogenase gene in GA-I patients by multiplex PCR and RFLP. The...
“adapter 1” of the Tn5 transposase, while the red symbol represents “adapter 2” of theTn5 transposase.cPCR amplification: PCR primer-1 and -2 are used to generate library for sequencing. Primer-1 and -2 are two universal PCR primers, which capture fragments with special length and add...
“Our research suggests that polymerase theta’s main function is to act as a reverse transcriptase,” says Dr. Pomerantz. “In healthy cells, the purpose of this molecule may be toward RNA-mediated DNA repair. In unhealthy cells, such as cancer cells, polymerase theta...
For pristine DNA templates ranged from 200pg to 12.5pg, the multiplex PCDR system consistently exhibited higher allele peak height as well as less allele dropout compared to the multiplex PCR references. Meanwhile, a significant reduction of stutter ratio was extensively observed in PCDR profiles. ...
4.1. Species Identification by Insertions/Deletions (SpinDel) Profiling The SpinDel protocol, developed by Pereira [53] uses a conventional genotyping methodology similar to that employed with short tandem repeats (STRs), involving multiplex PCR followed by fragment size determination done by capillary...