QIAGEN 替您完成 real-time PCR 引 物设计和反应优化 随着荧光定量 PCR 仪的普及,荧光定量 PCR 也开始飞入寻常实验室了.不过 real-time PCR 可不 像终端 PCR 那么轻巧.常规 PCR 只要扩出条带就算行,条带深点浅点偶尔有点副产物问题不太大,而 实时定量 PCR 既然目标是定量,自然就要严谨得多.稍有偏差都会...
The invention relates to a real-time PCR (Polymerase Chain Reaction) detection method of buffalo components in food and feed. The invention firstly discloses a primer for specific identification of buffalo components, and the primer can generate specific amplification of DNA (Deoxyribonucleic Acid) ...
Real-Time PCR In our quantitative realtime PCR section we offer you a selection of liquid master mixes and freeze-dried mixes, dual labelled probes and other supplements. All our master mixes contain in-house produced polymerase and nucleotides of the highest quality. Master mixes based on the ...
Real-time PCR master mixes, sometimes referred to as "mastermixes", "super mixes", or "ready mixes", are concentrated, pre-mixed solutions that contain many components required for a qPCR reaction, including buffer, enzymes, and dNTPs. With over 25 years of experience i...
qPCR Detection Kit is shipped at ambient temperature, But for long time storage, RT-QPCR Detection kit can be used within 12 mouths if the whole kit is stored at -20℃ i. Briefly (10-15 seconds) spin down all reagents. ii. For each 20µl PCR, mix the following components in a PC...
In this new era of digitalized workflows, Diomni Enterprise Software powerfully connects the components of your lab’s qPCR system in one single, easy-to-use solution. That’s the power of one. Play Mute Current Time 0:00 / Duration -:- Loaded...
Aiming at ribosome 18S rRNA gene sequence of apricot, the method designs the primer and probe for detecting apricot gene; and components of apricot in food and beverage are quantitatively detected by a real time PCR (Polymerase Chain Reaction) method. The real-time fluorescence PCR quantitative ...
If you do not have the needed Microsoft Visual C++ 2010 components, then the Aria installer will automatically install them to your PC when you initiate installation of the Aria software. 17 Agilent Aria Real-Time PCR Program Overview of the user interface Getting Started with the Aria Software...
The system combines a real-time PCR instrument with bespoke software and convenient pre-filled reagent components that, together with a single PCR protocol for a range of common targets and sample types, streamline the test workflow. The SureTect System is a unique solution combining speed and ...
4. PCR Reaction Setup: The PCR reaction mixture is prepared by combining the DNA template, primers, probes, and other components such as buffer, dNTPs, and DNA polymerase. The reaction mixture is then loaded into individual wells of a real-time PCR plate. 5. Thermal Cycling: The real-time...