Real-time PCRMediator probe PCR (MP PCR) is a novel detection format for real-time nucleic acid analysis. Label-free mediator probes (MP) and fluorogenic universal reporter (UR) oligonucleotides are combined to accomplish signal generation. Compared to conventional hydrolysis probe PCRs costs can ...
In general, relative expression changes by real-time RT-PCR were greater than those by microarray analysis, especially for genes that were markedly overexpressed. This may be explained by the better sensitivity and reliability of real-time RT-PCR compared to microarray, whose drawback is the ...
aUse of real-time PCR on faecal samples for detection of sub-clinical Salmonella infection in cattle did not improve the detection sensitivity compared to conventional bacteriology 对实时PCR的用途在粪便样品为临床症状不显的沙门氏菌传染的侦查在牛没有改进侦查敏感性与常规细菌学比较 [translate] ...
The principles of quantitative real-time PCR and melting curve analysis are explained. The quantification algorithms with internal and external standardization are derived mathematically, and potential pitfalls...
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On the other hand, four of the five phenotypes resistant to different concentrations of drugs had different expression levels for the 12 genes evaluated by real-time PCR. However, in the most resistant phenotype (TcR5x), the levels of mRNA from these 12 genes and seven more were similar to...
Development of real-time RT-PCR assays for eel gonadotropin subunits In the present work, we developed and validated qrtRT-PCR assays for relative quantification of European eel gonadotropin subunits: LHβ, FSHβ and GPα. As reference genes, we tested ARP, β-actin and cytb. Analysis of ser...
The output ofpcr_analyzeis explained in the documentation of the function?pcr_analyzeand the method it calls?pcr_ddct. Briefly, the input includes the CT value of c-mycnormalizedto the control GAPDH, Thecalibratedvalue of c-myc in the kidney relative to the brain samples and the finalrelati...
The overestimation of bacterial cell counts by real-time PCR observed in the stationary phase under higher-stress conditions might be explained by the accumulation of viable but nonculturable bacteria or dead bacteria and extracellular DNA. Considering these results, real-time PCR data collected from ...
a UDG, uracil-DNA-glycosylase; PCR, polymerase chain reaction. REAL-TIME PCR The two examples described above show that the reliability of in-house PCR assays for mycology and parasitology is currently not sufficient to allow their use in routine laboratories. The most significant changes expected...