real‐time PCRBackground and Objective Genotyping may be applied for rare blood group polymorphisms in a high-throughput mode as well for the molecular determination of blood groups due to unclear serological results. Material and Methods We developed and validated a DNA typing method for the ...
Real-time PCR fluorescent chemistries. There are more than a dozen formats available for the fluorescent detection of amplified DNA in kinetic (real-time) PCR. These chemistries are adaptable to... J Mackay,O Landt - 《Methods Mol Biol》 被引量: 49发表: 2007年 ...
In this study, we introduce a new, single-tube strategy that combines the tetra-primer ARMS PCR assay, SYBR Green I-based real-time PCR, and melting-point analysis with primer design strategies to detect the SNP of interest. This assay, T-Plex real-time PCR, is based on theTm...
Real-time PCR relies on highly specific and efficient amplification of the target sequence and the inclusion of fluorescent reporter molecules that enable the quantification of PCR products in real time. The use of a PCR primer specifically designed and validated for qPCR assays with your target ...
RTs catalyze the synthesis of a DNA copy (cDNA) of the target RNA molecules using a reverse transcription primer, dNTPs, and Mg2+ or Mn2+ as a cofactor. Reverse transcriptases have been adapted for use in a variety of in vitro applications including real-time and endpoint RT-PCR, labeled...
GE New Phantian 6000 digital photography system of the United States, and Hitachi 1000MA multifunctional digital gastrointestinal machine of Japan and BACTEC advanced instruments and equipment such as MGIT960 rapid bacterial culture instrument, real-time fluorescence quantitative PCR instrument, tuberculosis...
The real-time polymerase chain reaction (RT-PCR) is also called quantitative real-time polymerase chain reaction (QRT-PCR) or kinetic polymerase chain reaction (kPCR), which is a technique to simultaneously quantify the DNA molecules. It determines whether a specific DNA sequence is present in th...
关键词: Bacillus thuringiensis plasmid plasmid copy number(PCN quantitative real-time PCR 会议时间: 2009 被引量: 7497 收藏 引用 批量引用 报错 分享 全部来源 求助全文 merr.utm.my 相似文献 引证文献An introduction to MAMA (Meta-Analysis of MicroArray data) system Analyzing microarray data across ...
The basic real-time technique dates back to 1993, but it really took off in 1995-1996, when fluorescent techniques that monitor the accumulation of product, emerged. The theory of qPCR is not exactly like the practice. Theoretically, the number of templates double with each cycle of heating ...
Real-Time PCR concepts Real-time PCR —also known as quantitative reverse transcription PCR (RT-qPCR), and quantitative PCR (qPCR)—is one of the most powerful and sensitive gene analysis techniques available. It is used for a broad range of applications ...