Importantly, protein activities-based principal-component-analysis multivariate clusters analyzed for independent outcome prediction using Cox proportional-hazards model showed that protein゛ctivity (but not mutation-status) was independently predictive of outcome, demanding a paradigm-shift in patient﹕t...
regardless of mutation status, high-risk leukemic cells could only be killed using RAS‐inhibitor or PTPN11-inhibitor, but not PI3K/JAK‐inhibitors, suggesting a unified treatment target for up to 80% of DS‐ALL. Importantly, protein activities-based principal-component-analysis multivariate clusters ...
During mutational analysis, we found 14 of 39 samples (36%) harbored a mutation in a subset of genes coding for RAS-associating protein (Table1): including GEFs (40%), GAPs (25%), and effectors (35%) (Fig.2A). We found 20 mutations in 16 different genes encoding for a RAS-associa...
They observed that cancer cells expressing oncogenic Ras, an inner plasma membrane protein whose aberrant activation is associated with virtually all aspects of the malignant cancer phenotype, more highly utilize extracellular proteins as a source of amino acids to drive cellular growth [23,24]. ...
point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein. This antibody is predicted to react with H...
Human R-RAS protein mainly localizes to focal adhesions on the plasma membrane [6], where it regulates integrin functions by enhancing focal adhesion formation, cell adhesion, and cell spreading [7,8]. R-RAS2/TC21 localizes to the plasma membrane and the Golgi apparatus [9,10], suggesting...
Ras mutation, irrespective of cell type and p53 status, determines a cell's destiny to undergo apoptosis by okadaic acid, an inhibitor of protein phosphata... and Verma,A.K. (1999) Ras mutation, irrespective of cell type and p53 status, determines a cell's destiny to undergo apoptosis by...
The SH2 and SH3 domains of mammalian Grb2 couple the EGF receptor to the Ras activator mSosl Nature, 363 (1993), p. 83 View in ScopusGoogle Scholar 20 Egan SE, Giddings BW, Brooks MW, Buday L, Sizeland AM, Weinberg RA Association of Sos Ras exchange protein with Grb2 is implicated...
因子2 ( Ras protein activator like 2 ,RASAL2) 、GTPase 活化 蛋白 1 ( GTPase-activating protein 1,RASA1) 、IQ 样GTPase 活化蛋 白同源体 1 ( IQ motif containing GT— Pase.activating protein homologu e 1,IQGAP1) 、IQGAP2 等,其共同特点是都含有 1 个与Ras 蛋白结合区域— — ...
Previous analyses of K-Ras mutations in human tumours have consistently focussed on single-point mutations in codons 12, 13 and 61, where mutation has been shown to result in reduced Ras GAP GTPase activity, locking the protein in the active Ras-GTP conformation (Ellis and Clark, 2000), ...