Sigma-Aldrich公司的生命科学部门近日宣布推出增强版的OligoArchitect™,这一免费的在线工具可自动设计实时定量PCR分析的引物和探针。由行业标准的Beacon Designer™平台所支持,OligoArchitect成为一种可靠、方便的工具,与Sigma qPCR产品线互补。 这款软件的地址为:sigma.com/probedesignonline。目前分为在线版(Online)和...
TaqMan探针应指的是水解探针(hydrolysis probes)。 1.4 FRET probe FRET probe (fluorescence resonance energy transfer probe, 荧光共振能量转移探针)指的是一种机制,基于2个荧光基团的电子激发状态之间的相互作用的基础上的发光/淬火。LightCycler型探针指的是双杂交探针(d...
实时荧光定量聚合酶链式反应(qPCR)指的是在PCR进行的同时,对其过程进行监测的能力(即实时)。因此,数据可在PCR扩增过程中,而非PCR结束之后,进行收集。这为基于PCR的DNA和RNA定量方法带来了革命性的变革。在实时荧光定量PCR (qPCR)中,反应以循环中首次检测到目标扩增的时间点为特征,而非在一定循环数后目标...
PCR/qPCR/dPCR Assay DesignThe entire PCR workflow is vulnerable to factors which introduce variability. Many of the variable components are unavoidable, such as the source of the sample or the requirement for a reverse transcription step. Assay design is also highly variable and can make the dif...
这款软件的地址为:sigma.com/probedesignonline。目前分为在线版(Online)和咨询版(Consultative)。OligoArchitect™ Online v3.0除了可设计原先的双标记引物(水解探针)和SYBR Green I引物之外,现在还能设计Molecular Beacons、Scorpions™探针和LightCycler®探针。
当对实时荧光定量 PCR (qPCR) 的结果进行计算时,可以采用绝对或相对定量。 绝对定量 (数字 PCR 法)绝对定量(标准曲线法)相对定量 概述使用数字 PCR 进行绝对定量时,无需已知的标准品。能够以数字 PCR 重复数确定的精确度对目标分子直接进行定量。使用标准曲...
MGB Eclipse is a registered trademark of ELITech Group. Looking for design support? REQUEST CONSULT Create custom probes for any species with our free PrimerQuest™ Tool With PrimerQuest, you can: Optimize primer and probe designs for PCR, qPCR, or sequencing ...
Should I use probe- or dye-based detection for my qPCR assays? How should I design primers for Luna®qPCR? How long should my amplicon be for qPCR? Why is the Luna®qPCR Mix blue? Will this dye interfere with detection? Can I run the Luna®qPCR Mix on my qPCR instrument?
During the annealing cycle, the probe will anneal to its target sequence in- between the forward and reverse primer. As long as the probe is intact, the fluorescence of the reporter dye is quenched; however, when DNA polymerase extends the primer and replicates the template on which the Taq...
Determine your probe options When starting a new project, we recommend designing your assays using TaqMan QSY probes and the FAM, VIC, ABY, or JUN reporter dyes. See theTaqMan Multiplex Optimization guidefor design recommendations and advice on opti...