FailSafe™ PROBES Real-Time PCR Optimization Kit Greatly Improves Multiplex PerformanceReal-time quantitative PCR (qPCR) using fluorescently labeled nucleic acid probes, primers, or DNA-binding dyes, has become the method of choice for the detection and quantification ...
Black Hole Quencher 1 $333.00 USD $480.00 USD $726.00 USD * ZEN/Iowa Black™ FQ is a double-quenched probe, which provides superior performance compared to traditional single-quenched probes. For more information, download the PrimeTime Custom qPCR Probes Flyer.Product...
necessary. Specific consideration should be taken into account when designing primers and probes for multiplex qPCR and reverse transcription qPCR (RT-qPCR). This chapter provides guidelines for the design of suitable primers and probes and their subsequent validation through the development of singlex...
RealTime qPCR Design Tool—use to design primers, probes, and assays across exon boundaries for gene targets in species other than human, mouse, and rat. OligoAnalyzer Tool—use to analyze oligonucleotide melting temperature, hairpins, dimers, and mismatches. BLAST analysis can also be done di...
As this is a proof-of-concept study, we refrained from extensive optimization. Therefore, we increased the length of previously published STR loci primers instead of designing and optimizing primers from scratch. We describe the implementation of QueSTR probes in qPCR for forensic STR genotyping ...
TaqMan probes and qPCR primers are an optimal choice for real-time PCR research experiments that cannot be addressed with Thermo Fisher Scientific predesigned, ready-to-use TaqMan assays. Researchers can select from a collection of dual-labeled TaqMan probes and...
longitudinal disease tracking or early disease detection. The assay could be adapted for use with a variety of complex fluids, for example, saliva, urine and cerebrospinal fluid. With further optimization, this strategy could significantly reduce testing time, assay cost and sample volume, whilst inc...
LUXendin645+ (LUX+) and LUXendin645− (LUX−) cells were sorted for qPCR. g GLP1R, NKX6-1, INS, and GCG gene expression in sorted cells (n = 4 spheroids) (connecting bars indicate LUX+ and LUX− populations in the same samples) (paired Student’s t-test). LUXendin645 ...
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Functional antibody and epitope pairs were selected for further lateral-flow development and optimization to improve sensitivity. We validated expression of epitope candidates in both PURExpress and a crude extract (NEBExpress), but ultimately shifted to NEBExpress so that we could include the His epit...