FailSafe™ PROBES Real-Time PCR Optimization Kit Greatly Improves Multiplex PerformanceReal-time quantitative PCR (qPCR) using fluorescently labeled nucleic acid probes, primers, or DNA-binding dyes, has become the method of choice for the detection and quantification ...
PrimeTime Eco qPCR Probes The PrimeTime Eco qPCR Probe is the ideal scale when you need to perform ~500 reactions for gene expression analysis or genotyping. The combination of medium scale and low cost is ideal for initial screening of large sample sets. Eco scales are also amenable to dPCR...
RealTime qPCR Design Tool—use to design primers, probes, and assays across exon boundaries for gene targets in species other than human, mouse, and rat. OligoAnalyzer Tool—use to analyze oligonucleotide melting temperature, hairpins, dimers, and mismatches. BLAST analysis can also be done di...
Springer Nature is proud to announce ‘Methods Muse’, a new platform supporting experimental work by streamlining protocol design, implementation, validation, and optimization. Integrated with protocols.io ,Methods Muse offers prompt-based protocol generation and troubleshooting, thus saving valuable time,...
longitudinal disease tracking or early disease detection. The assay could be adapted for use with a variety of complex fluids, for example, saliva, urine and cerebrospinal fluid. With further optimization, this strategy could significantly reduce testing time, assay cost and sample volume, whilst inc...
RT-qPCR further verified the biomarker of apoptosis in positive groups. Then WB analysis found the expression of caspase-1 and GSDMD significantly enhanced in SLR20 treatment. Upregulation of CASP1 and CASP4 (encoding another mediator of pyroptosis, caspase-4) was seen in BT474 cells transfected...
LUXendin645+ (LUX+) and LUXendin645− (LUX−) cells were sorted for qPCR. g GLP1R, NKX6-1, INS, and GCG gene expression in sorted cells (n = 4 spheroids) (connecting bars indicate LUX+ and LUX− populations in the same samples) (paired Student’s t-test). LUXendin645 ...
Currently, the detection of single-stranded RNA (ssRNA) of SARS-CoV-2 [3] relies on hybridization-based molecular probes such as Taqman probe in reverse-transcription quantitative polymerase chain reaction (RT-qPCR) (Fig. 1A) [4], [5]. Alternative molecular probes for PCR-based methods were...
and 30 minutes incubation in the qPCR machine at 37° C. Then 30 data points are collected (30 seconds per data point). FIG. 32B: Average position-corrected endpoint fluorescence for the 8 M-Probes and targets. Signals from matched probes and targets are displayed along the main diagonal. ...
MeltMan: Optimization, Evaluation, and Universal Application of a qPCR System Integrating the TaqMan qPCR and Melting Analysis into a Single Assay. In the present work, we optimised and evaluated a qPCR system integrating 6-FAM (6-carboxyfluorescein)-labelled TaqMan probes and melting analysis using...