For different experimental requirements, TIANGEN has developed a series of real-time PCR products, including the SuperReal product series with dual enzyme system, the fast reaction FastFire series, the Talent qPCR PreMix for complex templates, as well as the FastKing One Step RT-qPCR kits for fa...
This application note illustrates how Liquidator 96 is used for both dye- and probe-based qPCR protocols while enhancing qPCR setup.
1. Probe-based Real-time qPCR Using a Standard 96-well Plate Platform cDNA synthesis (reverse transcription) on serum/plasma RNA samples for miRNA Calculate and take out 10 ng of RNA for each cDNA synthesis reaction. Add nuclease-free water to bring the final volume of 10 ng RNA to 1.67...
Coupled to a proper reverse transcription step, probe-based qPCR can be efficiently used for the analysis of the expression of difficult targets such as miRNAs. In this chapter, we describe the TaqMan advanced miRNA assay in which, owing to a poly(A)-tailing step, the reverse transcription ...
Type: Realtime-PCR (Probe-based qPCR) Catalog Number: EZ3304-R21 / EZ3304-R22 / EZ3304-R23 Pack Size: 1 ml / 5 ml / 25 ml Price: 150 USD / 425 USD / 1750 USD Promotion: 120 USD / 340 USD / 1400 USD (920 Customers Bought) MANUAL Description This kit is a dedicated premixed...
Amplification using quantitative PCR (qPCR) is measured by detecting changes in a fluorescent signal. Fluorescently labeled probes are frequently used as the fluorescent reporter in qPCR. The GoTaq(R) Probe qPCR and RT-qPCR Systems are ready-to-use 2X master mixes optimized for probe-based qPCR...
The quantitative polymerase chain reaction (qPCR) based assay is relatively easy and cheaper method that applies SyBr Green dye chemistry to measure telomere length. SyBr Green dye fluoresces after intercalation into the double stranded DNA (dsDNA), thus detection of unspecific products has been a ...
two types of universal fluorescence probes are used, and intermediate primers are introduced to guarantee specificity, so that the cost and duration of research and development for genotyping kits are significantly reduced, and UPIP-qPCR gives rise to the need for low cost and high accuracy of ...
Epidemiological studies of Pierce's disease (PD) can be confounded by a lack of taxonomic detail on the bacterial causative agent, Xf that typically colonize plants other than grape. Detection assays using ELISA and qPCR are effective at detecting and quantifying presence or absence, but offer no...
Further assessment with clinical samples showed that the developed ORFV exo RPA assay has good correlation with qPCR assays for detection of ORFV. These results suggest that the developed ORFV exo RPA assay is suitable for rapid detection of ORFV. The online version of this article (doi:...