Physiological Probes Answer PCR (polymerase chain reaction) is a widely used molecular biology technique that’s used for the amplification and detection of DNA and RNA sequences. RT-PCR, qPCR, and R-qPCR are all variations of the basic PCR technique. ...
Finally, because real-time qPCR reactions are run and data are evaluated in a unified, closed-tube qPCR system, opportunities for contamination are reduced and the need for postamplification manipulation is eliminated in qPCR analysis. Applications of Real-Time PCR/qPCR Assays Real-time PCR/qPCR ...
Typically, in a relative qPCR experiment designed to determine the fold change differences in gene expression, these will be a single gene of interest (target gene) and an endogenous control. TaqMan assays to detect the target and control genes, containing probes tha...
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The technique uses specific primers (short strands of nucleic acid needed to initiate DNA replication) and fluorescent probes to allow real-time quantification of target bacterial DNA during amplification. The qPCR assay is a mainstay of microbiological diagnostics within the National Health Service (...
If you are performing quantitative PCR (qPCR), then it’s crucial you understand what a Ctvalue is. We’ll take you through its critical role in qPCR, its varying names, how it’s calculated, and what it means when it all seems to go a bit wrong. ...
The technique uses specific primers (short strands of nucleic acid needed to initiate DNA replication) and fluorescent probes to allow real-time quantification of target bacterial DNA during amplification. The qPCR assay is a mainstay of microbiological diagnostics within the National Health Service (...
Genes identified in all types of transcriptomic analyses need to be further investigated using molecular approaches. RT-qPCR is the method of choice for evaluating the expression profiles of selected genes. Typically, at least three biological replicates are prepared to confirm differential transcript exp...
TaqMan® Mutation Detection Assays using castPCR™ TaqMan® Reagents & Kits Custom Primers & Probes Video: Introduction to the TaqMan® Assays QPCR Guarantee Program. White Papers Gene Expression Assay Performance Guaranteed With the TaqMan® Assays QPCR Guarantee Program ...
Quality control should be performed to ensure they are of the proper size and of sufficient concentration. This can include the use of automated electrophoresis instruments such as TapeStation or Fragment Analyzer for size characterization, as well as Qubit or qPCR-based assays for quantification. If...