What dyes are best for qPCR multiplexing? Below is a table of our instruments and the recommended dye combinations for multiplexing: Instrument Number of Filters and Dyes Available Passive Reference Dye ...
These tools contain design engines that use sophisticated formulas that, for example, take into account nearest neighbor analysis to calculate Tm, and allow you to begin your project sooner by easily analyzing and troubleshooting experimental designs. PrimerQuest Tool—use to generate highly customized ...
Choose from the convenient 0.5–2.5 nmol packaging size for digital PCR (dPCR) platforms Achieve high efficiency of probe-based qPCR under fast or standard cycling conditions Minimize troubleshooting─PrimeTime qPCR Probes are quality controlled via electrospray ionization mass spectrometry (ESI-MS)* and...
necessary. Specific consideration should be taken into account when designing primers and probes for multiplex qPCR and reverse transcription qPCR (RT-qPCR). This chapter provides guidelines for the design of suitable primers and probes and their subsequent validation through the development of singlex...
TaqMan 探针和 qPCR 引物 5’标记引物 Phosphoramidites 用于研究的高质量修饰核苷酸 预合成的 DNA 引物和寡核苷酸 寡核苷酸工具和应用中心 引物设计原则 Review options for custom-synthesized oligos, primers, probes, and genes for PCR, cloning, hybridization, real-time PCR, protein expression. Also, access...
Find resources and tips for getting started, and troubleshooting help all in one location. Service & Support FAQs & Protocols Contact Us * Open only to customers in the US (excluding Puerto Rico) and Canada. This offer will apply to qualifying orders received by Life Technologies no later than...
TaqMan Primers and Probes Support—Troubleshooting TaqMan Primers and Probes Support—Getting Started Find valuable information for your experiment using TaqMan primers and probes. Optimize your experiments...
Real-Time PCR Synthetic Biology Molecular Biology Education Support Centers Find resources and tips for getting started, and troubleshooting help all in one location. Service & Support FAQs & Protocols Contact Us 仅供科研使用,不可用于诊断目的。
TaqMan 探针和 qPCR 引物 5’标记引物 Phosphoramidites 用于研究的高质量修饰核苷酸 预合成的 DNA 引物和寡核苷酸 寡核苷酸工具和应用中心 引物设计原则 Review options for custom-synthesized oligos, primers, probes, and genes for PCR, cloning, hybridization, real-time PCR, protein expression. Also, access...
TaqMan 探针和 qPCR 引物 5’标记引物 Phosphoramidites 用于研究的高质量修饰核苷酸 预合成的 DNA 引物和寡核苷酸 寡核苷酸工具和应用中心 引物设计原则 Review options for custom-synthesized oligos, primers, probes, and genes for PCR, cloning, hybridization, real-time PCR, protein expression. Also, access...