Quantitative real-time PCR (qPCR) is a widely adopted technique used for scientific, clinical, diagnostic, or quality control purposes. One of the main applications of qPCR is gene expression analysis, although mutation detection, genotyping, DNA detection, and quantification (from pathogens or ...
since an accurate and reliable quantification depends on using efficient primers and probes. Design of primers and probes should meet several criteria to find potential primers and probes for specific qPCR assays. The formation of primer-
摘要: Design of primers and probes is one of the most crucial factors affecting the success and quality of quantitative real-time PCR (qPCR) analyses, since an accurate and reliable quantification depends...关键词: Quantitative real-time PCR Primers Probes Software and databases Validation Reverse...
Free PCR and qPCR design tools! IDT offers several free online tools (SciTools Web Tools) for oligonucleotide design and analysis. These tools contain design engines that use sophisticated formulas that, for example, take into account nearest neighbor analysis to calculate Tm, and allow you to be...
基因目标qPCR-Master用于定量2,使用特异性探针 短名称探针qPCR-Master(2x),用于定量实时PCR,两步实时RT-PCR,使用序列特异性探针。 technology Real time,RT-PCR,probe,step,PCR Alternative name probe quantification Real-time PCR test试剂Master Mix(2x)使用序列特异性探针测量体积实时PCR检测试剂和两步实时逆转录...
SYBR green Iand II: Bind to double-stranded DNA and are used in quantitative PCR (qPCR) for the detection and quantification of amplified DNA.SYBR Green I does not emit light on its own, but when it binds to newly synthesized double-stranded DNA, it undergoes a conformational change to fl...
Fig. 4: Multiplexed detection and quantification of miRNAs. a, Example current traces for barcodes without delay (i) and with delay (ii).b, Mean percentage delay ±50 nM miRNAs (two-tailedt-test, ****P = 3.58 × 10−4,n = 5,ntotal events = 76,841 (0 ...
Described in-houseqPCR method detected viral DNA in 5 of 20 specimens used. The results of this work show that developed HybProbes-based real-time PCR assay is very reliable and valuable for detection and quantification of equid herpesvirus type 2 DNA in different clinical samples. The high ...
We have designed an oligonucleotide probe for use in fluorescent in situ hybridization (FISH) and two pairs of PCR primers targeting a C. testosteroni subgroup. The FISH probe and one of the PCR primer pairs are suitable for quantification of C. testosteroni in mixed microbial communities using...
480 Probes Master is designed for real-time PCR using the LightCycler® 480 Instrument in combination with suitable probes (e.g., hydrolysis probes, Universal ProbeLibrary probes, and others) and gene-specific primers. The kit is ideally suited for hot start PCR assays for gene quantification...