Free PCR and qPCR design tools! IDT offers several free online tools (SciTools Web Tools) for oligonucleotide design and analysis. These tools contain design engines that use sophisticated formulas that, for example, take into account nearest neighbor analysis to calculate Tm, and allow you to be...
Design of primers and probes is one of the most crucial factors affecting the success and quality of quantitative real-time PCR (qPCR) analyses, since an accurate and reliable quantification depends on using efficient primers and probes. Design of …more Experimental...
quantification is crucial to provide a reliable laboratory tool for diagnosis of recent infections as well as for monitoring treatment efficacy.Methods/Principal FindingsWe have standardized and validated a multiplex Real-Time quantitative PCR assay (qPCR) based on TaqMan technology, aiming to quantify ...
锁核酸核苷酸可以被整合到qPCR探针中[1–4]。由于这些碱基显著提高了熔解温度(Tm),PrimeTime LNA qPCR探针可以设计得比标准qPCR探针更短。较短的探针具有更好的猝灭效果和更高的信噪比,因此更加灵敏。更重要的是,这些探针提供了改进的能力来区分突变或单核苷酸多态性(SNPs)[1]。可以设计一个锁核酸探针,使其ΔTm大...
Best of Both Worlds- New Dyes for qPCR used in combination with probes Neven Zoric Improving probe technology BEBO/BOXTO – a novel series of asymmetric cyanine dyes that bind to the minor groove of DNA 250-fold increase in fluorescence upon binding dsDNA N N S S N 1.20 1.00 0.80 In ...
A novel fluorescence-based assay for the rapid detection and quantification of cellular deoxyribonucleoside triphosphates. Nucleic Acids Res, 1(39):e112. RUO22-1594_002.1Author(s) Hans Packer, PhD, former Scientific Writer, IDT.Published Sep 16, 2013 Revised/updated Sep 10, 2023 Black Hole ...
Fig. 4: Multiplexed detection and quantification of miRNAs. a, Example current traces for barcodes without delay (i) and with delay (ii).b, Mean percentage delay ±50 nM miRNAs (two-tailedt-test, ****P = 3.58 × 10−4,n = 5,ntotal events = 76,841 (0 ...
480 Probes Master is designed for real-time PCR using the LightCycler® 480 Instrument in combination with suitable probes (e.g., hydrolysis probes, Universal ProbeLibrary probes, and others) and gene-specific primers. The kit is ideally suited for hot start PCR assays for gene quantification...
(B) EGFR mRNA expression in OC cell lines was detected by qPCR. (C) WB of EGFR protein in OC cell lines and (D) quantification analyses. (E) IF staining of EGFR in OC SKOV3 cells (positive) and A2780 cells (negative). EGFR, red; DAPI, blue. Magnification 400×; white scale bar...
S27). Target quantification was also achieved for each cycle with an R2 value> 0.9 as well as quantification limit of 0.01 fM utilizing the amplification step (Fig. 5F and G, Fig. S28A and B). Moreover, the dissipative probe was used to detect SARS-CoV-2 mutation D614G (Fig. 5...