Design of primers and probes is one of the most crucial factors affecting the success and quality of quantitative real-time PCR (qPCR) analyses, since an accurate and reliable quantification depends on using efficient primers and probes. Design of primers and probes should meet several criteria ...
Design of primers and probes is one of the most crucial factors affecting the success and quality of quantitative real-time PCR (qPCR) analyses, since an accurate and reliable quantification depends on using efficient primers and probes. Design of primers and probes should meet several criteria ...
摘要: Design of primers and probes is one of the most crucial factors affecting the success and quality of quantitative real-time PCR (qPCR) analyses, since an accurate and reliable quantification depends...关键词: Quantitative real-time PCR Primers Probes Software and databases Validation Reverse...
Real-time quantitative polymerase chain reaction (qPCR) has gained popularity as a technique to detect and quantify a specific group of target microorganisms from various environmental samples including soil, water, sediments, and sludge. Although qPCR is a very useful technique for nucleic acid quant...
Free PCR and qPCR design tools! IDT offers several free online tools (SciTools Web Tools) for oligonucleotide design and analysis. These tools contain design engines that use sophisticated formulas that, for example, take into account nearest neighbor analysis to calculate Tm, and allow you to be...
PrimeTime LNA qPCR 探针 ●可选择FAM、Cy® 3、Cy® 5、TEX、TYE™、YAK和HEX荧光基团。 ●提供满足大规模和高通量需求的合成规格(250nmol和1μmol)。 参考文献 1. Davialieva K, Kiprijanovska S, Plaseska-Karanfilska D.Fast, reliable and low cost user-developed protocol for detection, quanti...
Fig. 4: Multiplexed detection and quantification of miRNAs. a, Example current traces for barcodes without delay (i) and with delay (ii).b, Mean percentage delay ±50 nM miRNAs (two-tailedt-test, ****P = 3.58 × 10−4,n = 5,ntotal events = 76,841 (0 ...
Described in-houseqPCR method detected viral DNA in 5 of 20 specimens used. The results of this work show that developed HybProbes-based real-time PCR assay is very reliable and valuable for detection and quantification of equid herpesvirus type 2 DNA in different clinical samples. The high ...
480 Probes Master is designed for real-time PCR using the LightCycler® 480 Instrument in combination with suitable probes (e.g., hydrolysis probes, Universal ProbeLibrary probes, and others) and gene-specific primers. The kit is ideally suited for hot start PCR assays for gene quantification...
that the mere presence of a pathogen would imply that it is the causative agent of the current illness.5,14 There is a clear need to move towards quantitative multiplex diagnostics.21,80,81 qPCR allows better evaluation of the time course of the infection and of therapy monitoring than end-...