RealTime qPCR Design Tool—use to design primers, probes, and assays across exon boundaries for gene targets in species other than human, mouse, and rat. OligoAnalyzer Tool—use to analyze oligonucleotide melting temperature, hairpins, dimers, and mismatches. BLAST analysis can also be done di...
Does IDT offer VIC®probes for qPCR? How should I determine the annealing temperature for a probe-based qPCR assay? Should I select a melting temperature midway between my primer and probe melting temperatures (Tm)? What qPCR products does IDT ship at ambient temperature?
Biosearch Technologies is a trusted manufacturer of custom oligos and qPCR probes for research and a GMP service provider for clinical and diagnostic markets.
When it comes to assay design, we don’t believe in a one-dye-fits-all strategy. Our wide selection of dyes empowers you to design the best PCR or qPCR probe for your application without sacrificing performance. We have paired each dye with the BHQ quencher that offers optimal quenching ...
Design and order custom qPCR primers Set your assays up for success—the quality of the qPCR data is defined by the quality of the custom qPCR probes and primers chosen HPLC-purified probes—Removing impurities such as unconjugated dyes or truncated probes ...
What dyes are best for qPCR multiplexing? Below is a table of our instruments and the recommended dye combinations for multiplexing: Instrument Number of Filters and Dyes Available Passive Reference Dye Dyes Available ...
PAGE purification is not offered for post synthetic conjugation as yields are further decreased and certain modifications can be damaged during PAGE purification. All qPCR probes are available at a fixed price per oligonucleotide, within the following parameters:...
Design of primers and probes is one of the most crucial factors affecting the success and quality of quantitative real-time PCR (qPCR) analyses, since an accurate and reliable quantification depends on using efficient primers and probes. Design of …more Experimental...
Then, variable regions in the alignments were used to design fluorescent internal probes for qPCR aimed for a multiplex reaction in which relative abundance can be determined.Conclusion The authors propose this kind of cost-effective approach in the study of biofilms for the estimation of algae, ...
miR-126-5p and miR193b-3p. To further verify our results, we compared percentage delayed events with RT–qPCR of miR-29a in four samples. We found that our nanopore-based platform agreed with the RT–qPCR assay (Supplementary Fig.9)....