Selective proteomic proximity labeling assay using tyramide (SPPLAT): a quantitative method for the proteomic analysis of localized membrane-bound protein clusters. Current Protocols in Protein Science, 2015, 80 (19.27): 11-18Rees JS, Li XW, Perrett S, Lilley KS, Jackson AP. Selec- tive ...
Selective Proteomic Proximity Labeling Assay Using Tyramide (SPPLAT): A Quantitative Method for the Proteomic Analysis of Localized Membrane-Bound Protein Clusters.Selective Proteomic Proximity Labeling Assay Using Tyramide (SPPLAT): A Quantitative Method for the Proteomic Analysis of Localized Membrane-Boun...
Li, X.-W. et al. New insights into the DT40 B cell receptor cluster using a proteomic proximity labeling assay.J. Biol. Chem.289, 14434–14447 (2014). Paek, J. et al. Multidimensional tracking of GPCR signaling via peroxidase-catalyzed proximity labeling.Cell169, 338–349.e11 (2017)....
11). After performing CAT-S to label mitochondrial proteome in living cells, we collected the cells for proliferation assay and MMP measurement, to evaluate cellular and mitochondrial health, respectively. Gratifyingly, all three cell lines (HeLa, HEK293T, and K562) after CAT-S labeling stayed ...
To overcome some of the limitations of intracellular PDB for identification of AIS cell surface proteins, we used Selective Proteomic Proximity Labeling Assay Using Tyramide (SPPLAT)8,9; the approach has also been called Biotinylation by Antibody Recognition (BAR)10. Our application of this strategy...
allowingdigitalquantificationandinformationabouttheexactlocalizationofthetarget.ARevolutionintheDetectionofProteinsDuolink™enablesresearcherstostudysignallingpathwaysinnativecellsandtissueinrevolutionizingways.BasedonOlinkBioscience’sproprietaryinsituProximityLigationAssay(insituPLA),Duolink™makesitpossibletovisualize,...
We found that BirA(Arg118Lys) can mediate proximity labeling in cells under low biotin concentrations, and in vitro, can be inactivated by automodification at a high biotin concentration. Section snippets Expression, purification, and assay of BirA BirA proteins were expressed and purified as ...
Figure 1. Flowchart of the proximity labeling of SARS-CoV-2 proteins (A) The proteins encoded by the SARS-CoV-2 genome. (B) The workflow of the TurboID assay. To proximity label the proteins of SARS-CoV-2, all of the viral genes were cloned into mammalian expression vectors in frame ...
location in tissue samples offers exciting new opportunities in biomarker research. By providing the element of dual recognition to in situ analyses, pathology can expand into a new arena of analytes, beyond measuring the abundance of just a single protein.PLA™, a proximity ligation assay ...
P. Selective proteomic proximity labeling assay using tyramide (SPPLAT): a quantitative method for the proteomic analysis of localized membrane-bound protein clusters. Curr. Protoc. Protein Sci. 80, 19.27.1–19.27.18 (2015). Article Google Scholar Makowski, M. M., Willems, E., Jansen, P....