Protein-based approaches have relied on genetic, biochemical, and immunological analyses to identify the specificity of protective antibodies to polypeptide antigens. Genomics has also enabled the identification of new vaccine antigens. Once the complete sequence (or portions thereof) of the genomic DNA...
Urinary RBP excretion is correlated with HbA1c levels,[101, 123]urinary excretion of β2 microglobulin[97]and brush-border antigens,[98]but not with albumin excretion,[123]duration of diabetes,[97, 123]insulin dose, or blood pressure measurements.[97]Serum RBP levels were found to be increas...
We present proximity sequencing (Prox-seq) for simultaneous measurement of proteins, protein complexes and mRNAs in thousands of single cells. Prox-seq combines proximity ligation assay with single-cell sequencing to measure proteins and their complexes
protein. The E1 protein then converts to a double hexamer that encircles the DNA and unwinds the origin. The structure and function of the E1 protein is analogous to that of the polyomavirus SV40 T antigen. Much of our understanding about PV replication originates from key studies in BPV-1...
Protein-ligand interactions are essential for a variety of biological processes, such as interactions in antibody-antigen recognition [3], cell-cell communication [5], and signal transduction [6]. Abnormal interactions can lead to many diseases, so it is important to gain a thorough understanding ...
suppressing and normalizing mRNA GC content. To overcome its low immunogenicity, two engineered antigens were designed and synthesized as proteoliposomes: a human/mouse chimeric CLDN-5, and a CLDN-5-based artificial membrane protein consisting of symmetrically arranged ECRs. Intraperitoneal immunization...
Fig. 1. Analysis of protein abundances in human plasma using the Total Protein Approach. Whole plasma was processed using the multi-enzyme digestion FASP approach with strong anion exchange peptide fractionation before LC-MS/MS analysis as described in “Experimental Procedures.” Quantifications of ...
Although many of the ribosomal proteins are basic, the rRNP-P antigens (P0, P1, P2) are acidic phosphoproteins of 36, 19, and 17 kDa [252, 253]. The phosphoprotein moiety may account for observations that some high-affinity antibodies to dsDNA can also bind to these P proteins [254,...
Virus-coded cell surface antigens that appear soon after the infection of a cell by virus, but before virus replication has begun. Encoded by the so-called early genes. early gene See early antigens. early growth response genes A family of cys2-his2-type zinc-finger proteins; transcription...
Protein-Protein Interactions (PPIs) are fundamental means of functions and signalings in biological systems. The massive growth in demand and cost associated with experimental PPI studies calls for computational tools for automated prediction and underst