Protein-based approaches have relied on genetic, biochemical, and immunological analyses to identify the specificity of protective antibodies to polypeptide antigens. Genomics has also enabled the identification of new vaccine antigens. Once the complete sequence (or portions thereof) of the genomic DNA...
Gene set enrichment analysis was performed using GO-Elite (v.1.2.5) for human species68, which included Biological Process69, Molecular Function69, Cellular Component69, WikiPathways70, KEGG69and REACTOME71databases. Fisher’s exact test andZ-scores were used to test for significant enrichment amon...
[95]. hCGimmunoassaysmeasure the presence and/or the amount of this protein. Mostly they are based on the sandwich principal, which requires a target-protein-(antigen)-binding antibody and a second antibody against a distant epitope. The second antibody is labeled with anenzyme, a dye or ...
We present proximity sequencing (Prox-seq) for simultaneous measurement of proteins, protein complexes and mRNAs in thousands of single cells. Prox-seq combines proximity ligation assay with single-cell sequencing to measure proteins and their complexes
SOTIO Biotech a s Scientist SOTIO Biotech a s Scientist, P:reclinical Specifica an IQVIA business CSO Specifica VP & GM, IQVIA Lab Specifica an IQVIA business Head of Bioinformatics Specifica an IQVIA business Scientist, Bioinformatics Spectralys Biotech Managing Dir Analytics Sphere Fluidics Ltd...
Dendritic cell (DC)-tumor cell fusion vaccine Stimulates immune response against antigens present in both dendritic cells and cancer cells A DC-tumor cell fusion vaccine is made by fusing dendritic cells (immune cells that present antigens to other immune cells) with tumor cells. The fused ce...
forn = 3 replicates. ru relative units. Significant changes determined by unpairedt-test are indicated with asterisks *p-value < 0.05,n = 3 replicates.cProteases and cognate cleavage site sequences used to create additional regulatory MS2-CS-cNOT7 proteins.dAnalysis of MS2-CS-c...
Virus-coded cell surface antigens that appear soon after the infection of a cell by virus, but before virus replication has begun. Encoded by the so-called early genes. early gene See early antigens. early growth response genes A family of cys2-his2-type zinc-finger proteins; transcription...
and proteins secreted by cells into the extracellular matrix. Most of the methods utilize immunological detection of proteins by introducing antibodies to probe forspecific antigenson the target protein. These methods require lysing the tissue and cells and are therefore used as endpoint destructive ass...
Characterization of macrophage antigens profile recognized by serum of healthy donors and MN patients. Representative western blot analysis of full length gel of macrophage antigen profile. Recombinant FMNL1 (lane 3) and macrophage whole lysate (lanes 1–2, and 4–12) were separated by SDS-PAGE ...