In vitro validated antibody design against multiple therapeutic antigens using generative inverse folding Amir Shanehsazzadeh, Julian Alverio, George Kasun, Simon Levine, Jibran A Khan, Chelsea Chung, Nicolas Diaz, Breanna K Luton, Ysis Tarter, Cailen McCloskey, Katherine B Bateman, Hayley Carter...
(mAbs) produced using cell lines. The main objective is to determine monoclonal cell populations producing the desired mAb with a high degree of fidelity and activity for a specific antigen. The analysis of intact biologics is continued throug...
Their activation requires not only the direct stimulation of foreign antigens, but also the co-stimulus signal transmitted by the interaction of surface molecules.148 The CD40/CD40L pathway is one of the most important co-stimulating pathways in T-cell activation. Because of its critical role in...
The SpyTag/SpyCatcher protein ligation technology is valuable in many applications, but few methods exist for controlling reactivity. Here the authors report a method for controlling SpyCatcher activity via S-thiolation of engineered cysteines, demonstrating the value of their technology for the rapid ge...
Thus, EDN has been added to the growing list of alarmins,30 which are proteins that attract and enhance the activities of antigen-presenting cells, such as dendritic cells. Activation of cells through Toll-like receptor 2 (TLR2) further links the activity of EDN to components of innate ...
蛋白别名:5,6-dihydroxyindole-2-carboxylic acid oxidase; Catalase B; DHICA oxidase; Glycoprotein 75; Melanoma antigen gp75; MGC120175; MGC149362; RP11-3L8.1; TRP; TRY1; TRY4; TRYP1; Tyrosinase-related protein 1 基因别名:b-PROTEIN; CAS2; CATB; GP75; OCA3; TRP; ...
Briefly, heat-induced antigen retrieval was performed using 10 mM sodium citrate buffer, pH 6.5. Peroxidase activity was quenched with 3% H2O2 and tissues were blocked in 5% bovine serum albumin for 30 min. Primary antibody was added before incubating the sections overnight at 4 °C. ...
α-secretase targets APP within the Aβ domain that results in diminishing the formation of Aβ aggregates that is the key factor in the development of AD (Peron et al., 2018; Zhang et al., 2011). Therefore, it seems likely that upregulation of the activity of α-secretase boosts ...
p53 β-hydroxybutyrylation attenuates p53 activity. Cell Death Dis. 2019;10:243. Article PubMed PubMed Central Google Scholar Kang K, Lee S-R, Piao X, Hur GM. Post-translational modification of the death receptor complex as a potential therapeutic target in cancer. Arch Pharmacal Res. 2019...
It has been seen that a loss of function and lower stability occurs for many target proteins in standard sample buffer conditions, and it is essential to identify the components that are necessary to recover the integrity and activity of the protein [121]. The selection of solution conditions ...