Kollasch, Conor McMahon, Elana Simon, Chris Sander, Aashish Manglik, Andrew C. Kruse & Debora S. Marks Nature communications 12.1 (2021) • code::SeqDesign • mutation effect prediction • sequence generation • April 2021 Optimization of therapeutic antibodies by predicting antigen specificity...
(mAbs) produced using cell lines. The main objective is to determine monoclonal cell populations producing the desired mAb with a high degree of fidelity and activity for a specific antigen. The analysis of intact biologics is continued throug...
Simulations that capture antigen–antibody complexity Antigen–antibody prediction remains a complex computational challenge. Simulations with the new Absolut! package provide novel insights into the models and datasets tackling this problem. Pieter Meysman ...
The middle domains distinguish the different substrate proteins and regulate the activity of specific substrates of molecular chaperones.113 The C-terminal domain is a self-dimerization site of Hsp90, which enhances the interaction of two Hsp90 N-terminal domains.114 The current Hsp90 inhibitors can...
Thus, EDN has been added to the growing list of alarmins,30 which are proteins that attract and enhance the activities of antigen-presenting cells, such as dendritic cells. Activation of cells through Toll-like receptor 2 (TLR2) further links the activity of EDN to components of innate ...
the cofactor was an absolute requirement for reactivity as well as modulating the severity of the response[49]. The mechanism by which cofactors enable clinical reactivity is unclear. It has been suggested that effects on mucosal permeability by NSAIDs or exercise, resulting in higher antigen exposu...
Briefly, heat-induced antigen retrieval was performed using 10 mM sodium citrate buffer, pH 6.5. Peroxidase activity was quenched with 3% H2O2 and tissues were blocked in 5% bovine serum albumin for 30 min. Primary antibody was added before incubating the sections overnight at 4 °C. ...
activity,in vitromotility assays, cardiomyocyte unloaded shortening, and computer simulation approaches to determine how these N-terminal fragments affect muscle function. Overall, our studies demonstrate that ssMyBP-C and cMyBP-C share similar regulatory function at low Ca2+. Conversely, fsMyBP-C and...
reported that binding of the CD15 antigen, induced nuclear translocation of c-JUN and a significant increase in AP-1 DNA binding activity [104]. As c-JUN appears to be a crucial mediator in propagating and maintaining a malignant phenotype, this study suggests that these interactions are ...
et al. In vitro validated antibody design against multiple therapeutic antigens using generative inverse folding. In Generative AI and Biology (GenBio) Workshop, NeurIPS (2023). Olsen, T. H., Boyles, F. & Deane, C. M. Observed Antibody Space: a diverse database of cleaned, annotated, ...