Protein C activity assay kitPURPOSE:To obtain a kit, capable of measuring protein C activity in blood plasma with a high accuracy without requiring separation of the protein C from the blood plasma, by using a low-molecular weight thrombin inhibitor without a substance having a high molecular ...
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The Activated Protein C (APC, also known as PROC) Assay Kit is a 96-well format fluorogenic assay designed to measure the protease activity of Activated Protein C (PROC) for screening and profiling applications. Illustration of the assay principle. The substrate is an internally quenched fluorogen...
The activity and stability of PARIS-synthesized and solution-synthesized conjugates demonstrated that PARIS was an effective, rapid, and simple method to generate protein–polymer conjugates. Automation of PARIS significantly reduced synthesis/purification timelines, thereby opening a path to changing how ...
PDE6 activity assay PDE6 catalyzed hydrolysis of cGMP generates GMP and a proton, whose evolution changes the pH of buffer. The rate of this change was monitored in real-time using a fast response micro pH electrode (Hach-Lange, Germany)56. Activity of PDE6 (50 nM) was measured in 20...
(ProteinKinaseA)ActivityAssayKit Cat.No.KT-742 BACKGROUND PKAwasdiscoveredinthelaboratoryofEdwinG.Krebsinthe1960’s.Thisimportantclassofkinases,referedtoas Arg-directedkinasesorAGC-familykinases,includescAMP-dependentproteinkinase(PKAorcAPK),cGMP-dependent proteinkinase(PKG),proteinkinaseC,AktandRSK.These...
No detectable activity under the conditions of the assay. c Anticoagulant activity of S360A-APC is not due to proteolysis of factor Va and, in contrast to rwt-APC, is independent of the incubation time of APC with the plasma (19, 26). d ND, not determined. e Relative activity derived ...
Protein kinase C is a complex family of isoenzymes that affect signal transduction, resulting in modulation of proliferation, apoptosis, and cellular differentiation [84,85]. Protein kinase C activity can be inhibited by staurosporine, bryostatin, or antisense oligonucleotides [84,85]. Inhibition of ...
C.-G.W. and F.H. performed TRUPATH assays. J.M.M. conceived, optimized and performed in vitro cellular G-protein activation and signalling mutagenesis assays and interpreted results. M.M.P.-S. performed the GTPγS activity assay and analysed the data. T.-J.G. collected and analysed ...
Assessing the residual antibacterial activity of clinical materials disinfected with glutaraldehyde, o-phthalaldehyde, hydrogen peroxide or 2-bromo-2-nitro-1,3-propanediol by means of a bacterial toxicity assay. Clin. Microbiol. Infect. 10, 984–989 (2004). Article CAS PubMed Google Scholar Tung...