Protein-glutaminase (PG) catalyzes the deamidation of only the side-chain amide group of glutamine (Gln) residues in proteins, leading to improved protein functionalities such as water solubility, emulsifying properties, and foaming ability. However, the application of PG to food processing is ...
Of which, eleven candidates were cloned for construction of expression vectors to mediate the expression of Chryseobacterium proteolyticum protein glutaminase (PGA). The signal peptide of GlmU was confirmed that it significantly mediated PGA secretion into media with the maximum activity of 0.16 U/...
they should not be blamed at first glance, because, it might be the result of their adaptive failure towards natural field conditions as well as local edaphic factors. Instead, efforts should be made to develop such technologies which can monitor the real-time performance...
How protein synthesis capacity and activity change during a T cell immune response Regulators of the protein synthesis rate in T cells How protein degradation changes during a T cell immune response T cell energy metabolism—what have we learned from proteomics studies? Crosstalk between glucose metab...
h Mitochondrial ACO2 activity was analyzed by the in-gel activity assay. Immunoblot analyses were performed with the indicated antibodies. i PaTu-8988t cells were transfected with a control siRNA, ACO2 siRNA, or DLST siRNA. α-KG levels were determined and normalized to the numbers of cells ...
Glutamine uptake, that is, glutaminase (GLS) activity, was upregulated in LoVo CDDP R cells. Furthermore, bioinformatics analysis indicated that the 3' UTR of GLS1 contained a putative binding motif of YTHDF1, and an interaction was further validated by a protein-RNA interaction assay (RNA ...
2024, Analytical Methods l-Glutamine-, peptidyl- and protein-glutaminases: structural features and applications in the food industry 2022, World Journal of Microbiology and Biotechnology Keeping Cell Death Alive: An Introduction into the French Cell Death Research Network 2022, BiomoleculesView...
Therefore, we performed a third enzymatic activity assay on known SIRT5-targeted peptides. We carried out an in vitro lysine deglutarylation reaction using the synthetic Kglu peptide, VKSKgluATNLWW, whose sequence is from DNA starvation/stationary phase protection (Dps) protein identified in our...
glutaminase-man<0.572 9. asparaginase uricaseman<3810. glutaminase-mouse22411. asparaginase asparaginasemouse<69612. arginasemouse<11213. suproxidemouse0.0616.514. dismutase lactoferrinmouse0.05114. streptokinasemouse0.070.3315. plasma-mouse0.050.2215. ...
The biological activity of the fractions was normalised with respect to the protein by means of the radioactivity (using I125 labelled gm-CSF, Amersham). Fractions ranged from no biological activity to 3-6 times the activity seen in fractions from the peak of unmodified material from an identica...